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dc.contributor.authorPatil, Rutuja H.
dc.contributor.authorKotta-Loizou, Ioly
dc.contributor.authorPalyzová, Andrea
dc.contributor.authorPluháček, Tomáš
dc.contributor.authorCoutts, Robert H. A.
dc.contributor.authorStevens, David A.
dc.contributor.authorHavlíček, Vladimír
dc.date.accessioned2021-08-24T14:31:48Z
dc.date.available2021-08-24T14:31:48Z
dc.date.issued2021-06-22
dc.identifier.citationPatil , R H , Kotta-Loizou , I , Palyzová , A , Pluháček , T , Coutts , R H A , Stevens , D A & Havlíček , V 2021 , ' Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics ' , Journal of Fungi , vol. 7 , no. 7 , e497 . https://doi.org/10.3390/jof7070497
dc.identifier.issn2309-608X
dc.identifier.otherPURE: 25819130
dc.identifier.otherPURE UUID: 68491e17-5a46-45ab-9093-b7acd1339724
dc.identifier.otherJisc: 9320bf7860ed4985bd3c3f97ba652211
dc.identifier.urihttp://hdl.handle.net/2299/25018
dc.description.abstractA virus-free (VF) A. fumigatus isolate has been shown to be resistant in competition with Pseudomonas as compared to the isogenic line infected with Aspergillus fumigatus polymycovirus 1 (AfuPmV-1), and this phenotype was apparently related to alterations in iron metabolism. Here we investigated further the mechanisms underpinning this phenotype. The extracellular siderophore profiles of five isogenic VF and virus-infected (VI) strains were sampled at 24, 31, 48, 54, and 72 h in submerged cultures and quantitatively examined by liquid chromatography and mass spectrometry. Intracellular profiles of conidia and cultures at the stationary growth phase were defined. VF A. fumigatus demonstrated the best fitness represented by the fastest onset of its exponential growth when grown on an iron-limited mineral medium. The exponential phase and transitional production phase of the extracellular triacetylfusarinine C (TafC) were achieved at 24 and 31 h, respectively, contrary to VI strains, which acted more slowly. As a result, the TafC reservoir was consumed sooner in the VF strain. Additionally, the VF strain had lower ferricrocin and higher hydroxyferricrocin content in the pellet during the stationary phase. All of these differences were significant (Kruskal–Wallis, p 0.01). In our study, the siderophore reservoir of a VF strain was consumed sooner, improving the fitness of the VF strain in competition with P. aeruginosa.en
dc.language.isoeng
dc.relation.ispartofJournal of Fungi
dc.rightsOpen
dc.subjectAspergillus fumigatus
dc.subjectintermicrobial competition
dc.subjectpolymycovirus
dc.subjectPseudomonas aeruginosa
dc.subjectsiderophore
dc.titleFreeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tacticsen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Agriculture, Food and Environmental Management Research
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2021-06-22
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.3390/jof7070497
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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