dc.contributor.author | Furber, Matthew | |
dc.contributor.author | Pyle, Simone | |
dc.contributor.author | Roberts, Michael | |
dc.contributor.author | Roberts, Justin | |
dc.date.accessioned | 2021-12-13T10:30:03Z | |
dc.date.available | 2021-12-13T10:30:03Z | |
dc.date.issued | 2021-12-08 | |
dc.identifier.citation | Furber , M , Pyle , S , Roberts , M & Roberts , J 2021 , ' Comparing Acute, High Dietary Protein and Carbohydrate Intake on Transcriptional Biomarkers, Fuel Utilisation and Exercise Performance in Trained Male Runners ' , Nutrients , vol. 13 , no. 12 , e4391 . https://doi.org/10.3390/nu13124391 | |
dc.identifier.issn | 2072-6643 | |
dc.identifier.other | Jisc: 5fb849685d7c4798b0fc96a2b72952d3 | |
dc.identifier.uri | http://hdl.handle.net/2299/25244 | |
dc.description | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) | |
dc.description.abstract | Manipulating dietary macronutrient intake may modulate adaptive responses to exercise, and improve endurance performance. However, there is controversy as to the impact of short-term dietary modification on athletic performance. In a parallel-groups, repeated measures study, 16 trained endurance runners (maximal oxygen uptake (V˙O2max): 64.2 ± 5.6 mL·kg−1·min−1) were randomly assigned to, and provided with, either a high-protein, reduced-carbohydrate (PRO) or a high-carbohydrate (CHO) isocaloric-matched diet. Participants maintained their training load over 21-consecutive days with dietary intake consisting of 7-days habitual intake (T1), 7-days intervention diet (T2) and 7-days return to habitual intake (T3). Following each 7-day dietary period (T1−T3), a micro-muscle biopsy was taken for assessment of gene expression, before participants underwent laboratory assessment of a 10 km treadmill run at 75% V˙O2max, n treadmill run at 70% vV˙O2max time to exhaustion (TTE) trial. The PRO diet resulted in a modest change (1.37-fold increase, p = 0.016) in AMPK expression, coupled with a significant increase in fat oxidation (0.29 ± 0.05 to 0.59 ± 0.05 g·min−1, p 0.0001). However, a significant reduction of 23.3% (p = 0.0003) in TTE post intervention was observed; this reverted back to pre levels following a return to the habitual diet. In the CHO group, whilst no change in sub-maximal fuel utilisation occurred at T2, a significant 6.5% increase in TTE performance (p = 0.05), and a modest, but significant, increase in AMPK (p = 0.042) and PPAR (p = 0.029) mRNA expression compared to T1 were observed; with AMPK (p = 0.011) and PPAR (p = 0.044) remaining significantly elevated at T3. In conclusion, a 7-day isocaloric high protein diet significantly compromised high intensity exercise performance in trained runners with no real benefit on gene markers of training adaptation. A significant increase in fat oxidation during submaximal exercise was observed post PRO intervention, but this returned to pre levels once the habitual diet was re-introduced, suggesting that the response was driven via fuel availability rather than cellular adaptation. A short-term high protein, low carbohydrate diet in combination with endurance training is not preferential for endurance running performance. | en |
dc.format.extent | 15 | |
dc.format.extent | 1079036 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nutrients | |
dc.subject | protein | |
dc.subject | AMPK | |
dc.subject | PGC-1α | |
dc.subject | substrate oxidation | |
dc.subject | dietary intake | |
dc.subject | endurance performance | |
dc.subject | runners | |
dc.title | Comparing Acute, High Dietary Protein and Carbohydrate Intake on Transcriptional Biomarkers, Fuel Utilisation and Exercise Performance in Trained Male Runners | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Psychology | |
dc.contributor.institution | Centre for Research in Psychology and Sports | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.3390/nu13124391 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |