dc.contributor.author | Wilkes, Thomas | |
dc.contributor.author | Warner, Douglas | |
dc.date.accessioned | 2021-12-15T14:20:58Z | |
dc.date.available | 2021-12-15T14:20:58Z | |
dc.date.issued | 2021-11-30 | |
dc.identifier.citation | Wilkes , T & Warner , D 2021 , ' An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi ' , Access Microbiology , vol. 3 , no. 11 , 000292 . https://doi.org/10.1099/acmi.0.000292 | |
dc.identifier.issn | 2516-8290 | |
dc.identifier.other | ORCID: /0000-0002-9136-9713/work/104970477 | |
dc.identifier.uri | http://hdl.handle.net/2299/25262 | |
dc.description | © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/ | |
dc.description.abstract | Sheaffer blue ink is an effective method to stain arbuscular mycorrhizal (AM) fungi in a variety of plant species. It has, however, received criticism for its potential rapid degradation and short-term viability. The long and medium term storage and viability of stained samples has not, to date, been described for this particular staining method. This short communication reports on the viability of 730 samples stained with Sheaffer® blue ink stored for the duration of four years in microscope slide boxes out of direct sunlight. There was no significant difference in micrograph image quality and presence of stain between years as indicated by the number of AM fungal structures quantified. In conclusion Sheaffer® blue ink stain does not deteriorate in the medium term. | en |
dc.format.extent | 4 | |
dc.format.extent | 937541 | |
dc.language.iso | eng | |
dc.relation.ispartof | Access Microbiology | |
dc.subject | arbuscular mycorrhizal fungi | |
dc.subject | blue ink stain | |
dc.subject | root cortex | |
dc.subject | storage | |
dc.subject | degradation | |
dc.title | An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Agriculture and Environment Research Unit | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Agriculture and Environmental Management Research | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1099/acmi.0.000292 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |