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        Developing microfluidic devices and techniques for isolation and detection of Extracellular Vesicles (EVs)

        Author
        Lekkala, Himayasri
        Dimov, Nikolay
        Johnston, Ian
        Inal, Jameel
        Attention
        2299/25442
        Abstract
        The purpose of this research is to develop microfluidic devices and systems for the effective isolation of bacterial Extracellular vesicles (EVs) by applying Tangential Flow Filtration (TFF) operation. The purification and maximum quantification of these vesicles is achieved in three stages of TFF operation. The downstream analysis and characterization of isolated EVs is performed using Nanoparticle Tracking Analysis (NTA). Developing microfluidic systems and techniques for Extracellular vesicle isolation based on their size variation can overcome the issues of EV analysis from a complex bacterial culture which can also bridge the gap between research and clinical services.
        Publication date
        2020-10-09
        Other links
        http://hdl.handle.net/2299/25442
        Relations
        School of Physics, Engineering & Computer Science
        School of Life and Medical Sciences
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