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dc.contributor.authorBurton, Shawn D
dc.contributor.authorBrown, Audrey
dc.contributor.authorEiting, Thomas P
dc.contributor.authorYoungstrom, Isaac A
dc.contributor.authorRost, Thomas C
dc.contributor.authorSchmuker, Michael
dc.contributor.authorWachowiak, Matt
dc.date.accessioned2022-09-12T11:00:03Z
dc.date.available2022-09-12T11:00:03Z
dc.date.issued2022-07-21
dc.identifier.citationBurton , S D , Brown , A , Eiting , T P , Youngstrom , I A , Rost , T C , Schmuker , M & Wachowiak , M 2022 , ' Mapping odorant sensitivities reveals a sparse but structured representation of olfactory chemical space by sensory input to the mouse olfactory bulb ' , Elife , vol. 11 , e80470 . https://doi.org/10.7554/eLife.80470
dc.identifier.issn2050-084X
dc.identifier.otherJisc: 505077
dc.identifier.urihttp://hdl.handle.net/2299/25761
dc.description© 2022, Burton et al. This article is distributed under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/
dc.description.abstractIn olfactory systems, convergence of sensory neurons onto glomeruli generates a map of odorant receptor identity. How glomerular maps relate to sensory space remains unclear. We sought to better characterize this relationship in the mouse olfactory system by defining glomeruli in terms of the odorants to which they are most sensitive. Using high-throughput odorant delivery and ultrasensitive imaging of sensory inputs, we imaged responses to 185 odorants presented at concentrations determined to activate only one or a few glomeruli across the dorsal olfactory bulb. The resulting datasets defined the tuning properties of glomeruli - and, by inference, their cognate odorant receptors - in a low-concentration regime, and yielded consensus maps of glomerular sensitivity across a wide range of chemical space. Glomeruli were extremely narrowly tuned, with ~25% responding to only one odorant, and extremely sensitive, responding to their effective odorants at sub-picomolar to nanomolar concentrations. Such narrow tuning in this concentration regime allowed for reliable functional identification of many glomeruli based on a single diagnostic odorant. At the same time, the response spectra of glomeruli responding to multiple odorants was best predicted by straightforward odorant structural features, and glomeruli sensitive to distinct odorants with common structural features were spatially clustered. These results define an underlying structure to the primary representation of sensory space by the mouse olfactory system.en
dc.format.extent6062326
dc.language.isoeng
dc.relation.ispartofElife
dc.subjectOlfactory Bulb/physiology
dc.subjectAnimals
dc.subjectSmell/physiology
dc.subjectMice
dc.subjectOlfactory Receptor Neurons/physiology
dc.subjectReceptors, Odorant/metabolism
dc.subjectOdorants
dc.subjectchemoinformatics
dc.subjectolfactometry
dc.subjectodor
dc.subjectimaging
dc.subjectcoding
dc.subjectNeuroscience
dc.subjectMouse
dc.subjectResearch Article
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Immunology and Microbiology
dc.subjectGeneral Neuroscience
dc.titleMapping odorant sensitivities reveals a sparse but structured representation of olfactory chemical space by sensory input to the mouse olfactory bulben
dc.contributor.institutionCentre of Data Innovation Research
dc.contributor.institutionSchool of Physics, Engineering & Computer Science
dc.contributor.institutionDepartment of Computer Science
dc.contributor.institutionBiocomputation Research Group
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85135561283&partnerID=8YFLogxK
rioxxterms.versionofrecord10.7554/eLife.80470
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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