dc.contributor.author | Thoma, Anastasia | |
dc.contributor.author | Earl, Kate E. | |
dc.contributor.author | Goljanek‐Whysall, Katarzyna | |
dc.contributor.author | Lightfoot, Adam P. | |
dc.date.accessioned | 2022-11-28T14:15:03Z | |
dc.date.available | 2022-11-28T14:15:03Z | |
dc.date.issued | 2022-11-25 | |
dc.identifier.citation | Thoma , A , Earl , K E , Goljanek‐Whysall , K & Lightfoot , A P 2022 , ' Major histocompatibility complex I‐induced endoplasmic reticulum stress mediates the secretion of pro‐inflammatory muscle‐derived cytokines ' , Journal of Cellular and Molecular Medicine . https://doi.org/10.1111/jcmm.17621 | |
dc.identifier.issn | 1582-1838 | |
dc.identifier.other | Jisc: 745758 | |
dc.identifier.other | publisher-id: jcmm17621 | |
dc.identifier.other | society-id: jcmm-07-2022-006.r2 | |
dc.identifier.uri | http://hdl.handle.net/2299/25921 | |
dc.description | © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article distributed under the Creative Commons Attribution License, to view a copy of the license, see: This is an open access article under the terms of the Creative Commons Attribution License,https://creativecommons.org/licenses/by/4.0/ | |
dc.description.abstract | Major histocompatibility complex (MHC) I is an important component of intracellular antigen presentation. However, improper expression of MHC I upon the cell surface has been associated with several autoimmune diseases. Myositis is a rare acquired autoimmune disease which targets skeletal muscle, and MHC I overexpression on the surface of muscle fibres and immune cell infiltration are clinical hallmarks. MHC I overexpression may have an important pathogenic role, mediated by the activation of the endoplasmic reticulum (ER) stress response. Given the evidence that muscle is a diverse source of cytokines, we aimed to investigate whether MHC I overexpression can modify the profile of muscle‐derived cytokines and what role the ER stress pathway may play. Using C2C12 myoblasts we overexpressed MHC I with a H‐2kb vector in the presence or absence of salubrinal an ER stress pathway modifying compound. MHC I overexpression induced ER stress pathway activation and elevated cytokine gene expression. MHC I overexpression caused significant release of cytokines and chemokines, which was attenuated in the presence of salubrinal. Conditioned media from MHC I overexpressing cells induced in vitro T‐cell chemotaxis, atrophy of healthy myotubes and modified mitochondrial function, features which were attenuated in the presence of salubrinal. Collectively, these data suggest that MHC I overexpression can induce pro‐inflammatory cytokine/chemokine release from C2C12 myoblasts, a process which appears to be mediated in‐part by the ER stress pathway. | en |
dc.format.extent | 10 | |
dc.format.extent | 2401748 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Cellular and Molecular Medicine | |
dc.subject | ORIGINAL ARTICLE | |
dc.subject | ORIGINAL ARTICLES | |
dc.subject | ER stress | |
dc.subject | major histocompatibility complex (MHC) I | |
dc.subject | myokines | |
dc.subject | skeletal muscle | |
dc.title | Major histocompatibility complex I‐induced endoplasmic reticulum stress mediates the secretion of pro‐inflammatory muscle‐derived cytokines | en |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Agriculture, Food and Environmental Management Research | |
dc.contributor.institution | Food Policy, Nutrition and Diet | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1111/jcmm.17621 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |