dc.contributor.author | Hutter, Victoria | |
dc.contributor.author | Hopper, Sarah | |
dc.contributor.author | Skamarauskas, John | |
dc.contributor.author | Hoffman, Ewelina | |
dc.date.accessioned | 2022-11-28T15:15:02Z | |
dc.date.available | 2022-11-28T15:15:02Z | |
dc.date.issued | 2023-02-28 | |
dc.identifier.citation | Hutter , V , Hopper , S , Skamarauskas , J & Hoffman , E 2023 , ' High content analysis of in vitro alveolar macrophage responses can provide mechanistic insight for inhaled product safety assessment ' , Toxicology in Vitro , vol. 86 , 105506 . https://doi.org/10.1016/j.tiv.2022.105506 | |
dc.identifier.issn | 0887-2333 | |
dc.identifier.other | ORCID: /0000-0002-7998-924X/work/124034552 | |
dc.identifier.uri | http://hdl.handle.net/2299/25922 | |
dc.description | © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license. https://creativecommons.org/licenses/by/4.0/ | |
dc.description.abstract | Assessing the safety of inhaled substances in the alveolar region of the lung requires an understanding of how the respired material interacts with both physical and immunological barriers. Human alveolar-like macrophages in vitro provide a platform to assess the immunological response in the airways and may better inform the understanding of a response to an inhaled challenge being adaptive or adverse. The aim of this study was to determine if a morphometric phenotyping approach could discriminate between different inhaled nicotine products and indicate the potential mechanism of toxicity of a substance. Cigarette smoke (CS) and e-liquids extracted into cell culture medium were applied to human alveolar-like macrophages in mono-culture (ImmuONE™) and co-culture (ImmuLUNG™) to test the hypothesis. Phenotype profiling of cell responses was highly reproducible and clearly distinguished the different responses to CS and e-liquids. Whilst the phenotypes of untreated macrophages were similar regardless of culture condition, macrophages cultured in the presence of epithelial cells were more sensitive to CS-induced changes related to cell size and vacuolation processes. This technique demonstrated phenotypical observations typical for CS exposure and indicative of the established mechanisms of toxicity. The technique provides a rapid screening approach to determine detailed immunological responses in the airways which can be linked to potentially adverse pathways and support inhalation safety assessment. | en |
dc.format.extent | 10 | |
dc.format.extent | 7448121 | |
dc.language.iso | eng | |
dc.relation.ispartof | Toxicology in Vitro | |
dc.subject | Alveolar macrophage | |
dc.subject | E-liquid | |
dc.subject | High content analysis | |
dc.subject | In vitro | |
dc.subject | Inhalation toxicology | |
dc.subject | Toxicology | |
dc.title | High content analysis of in vitro alveolar macrophage responses can provide mechanistic insight for inhaled product safety assessment | en |
dc.contributor.institution | Centre for Research into Topical Drug Delivery and Toxicology | |
dc.contributor.institution | Pharmaceutics | |
dc.contributor.institution | Airway Group | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Basic and Clinical Science Unit | |
dc.contributor.institution | Centre for Health Services and Clinical Research | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85140878292&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1016/j.tiv.2022.105506 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |