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dc.contributor.authorRossi , Izadora Volpato
dc.contributor.authorNunes, Maria Alice Ferreira
dc.contributor.authorSabatke, Bruna
dc.contributor.authorRibas , Hennrique Taborda
dc.contributor.authorWinnischofer , Sheila Maria Brochado
dc.contributor.authorRamos, Augusto Savio Peixoto
dc.contributor.authorRamirez, Marcel Ivan
dc.contributor.authorInal, Jameel
dc.date.accessioned2023-01-04T14:00:02Z
dc.date.available2023-01-04T14:00:02Z
dc.date.issued2022-12-14
dc.identifier.citationRossi , I V , Nunes , M A F , Sabatke , B , Ribas , H T , Winnischofer , S M B , Ramos , A S P , Ramirez , M I & Inal , J 2022 , ' An induced population of Trypanosoma cruzi epimastigotes more resistant to complement lysis promotes a phenotype with greater differentiation, invasiveness, and release of extracellular vesicles ' , Frontiers Cellular and Infection Microbiology . https://doi.org/10.3389/fcimb.2022.1046681
dc.identifier.issn2235-2988
dc.identifier.urihttp://hdl.handle.net/2299/25978
dc.description© 2022 Rossi, Nunes, Sabatke, Ribas, Winnischofer, Ramos, Inal and Ramirez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/
dc.description.abstractIntroduction: Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi, which uses blood-feeding triatomine bugs as a vector to finally infect mammalian hosts. Upon entering the host, the parasite needs to effectively evade the attack of the complement system and quickly invade cells to guarantee an infection. In order to accomplish this, T. cruzi expresses different molecules on its surface and releases extracellular vesicles (EVs). Methods: Here, we have selected a population of epimastigotes (a replicative form) from T. cruzi through two rounds of exposure to normal human serum (NHS), to reach 30% survival (2R population). This 2R population was characterized in several aspects and compared to Wild type population. Results: The 2R population had a favored metacyclogenesis compared with wild-type (WT) parasites. 2R metacyclic trypomastigotes had a two-fold increase in resistance to complementmediated lysis and were at least three times more infective to eukaryotic cells, probably due to a higher GP82 expression in the resistant population. Moreover, we have shown that EVs from resistant parasites can transfer the invasive phenotype to the WT population. In addition, we showed that the virulence phenotype of the selected population remains in the trypomastigote form derived from cell culture, which is more infective and also has a higher rate of release of trypomastigotes from infected cells. Conclusions: Altogether, these data indicate that it is possible to select parasites after exposure to a particular stress factor and that the phenotype of epimastigotes remained in the infective stage. Importantly, EVs seem to be an important virulence fator increasing mechanism in this context of survival and persistence in the host.en
dc.format.extent12
dc.format.extent4715462
dc.language.isoeng
dc.relation.ispartofFrontiers Cellular and Infection Microbiology
dc.titleAn induced population of Trypanosoma cruzi epimastigotes more resistant to complement lysis promotes a phenotype with greater differentiation, invasiveness, and release of extracellular vesiclesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.3389/fcimb.2022.1046681
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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