dc.contributor.author | Etifa, Perezimor | |
dc.contributor.author | Rodríguez, César | |
dc.contributor.author | Harmanus, Céline | |
dc.contributor.author | Sanders, Ingrid M. J. G. | |
dc.contributor.author | Sidorov, Igor A. | |
dc.contributor.author | Mohammed, Olufunmilayo A. | |
dc.contributor.author | Savage, Emily | |
dc.contributor.author | Timms, Andrew R. | |
dc.contributor.author | Freeman, Jane | |
dc.contributor.author | Smits, Wiep Klaas | |
dc.contributor.author | Wilcox, Mark H. | |
dc.contributor.author | Baines, Simon D. | |
dc.contributor.editor | Monchi, Mehran | |
dc.contributor.editor | Samarkos, Michael | |
dc.contributor.editor | Di Bella, Stefano | |
dc.date.accessioned | 2023-03-07T14:30:02Z | |
dc.date.available | 2023-03-07T14:30:02Z | |
dc.date.issued | 2023-02-22 | |
dc.identifier.citation | Etifa , P , Rodríguez , C , Harmanus , C , Sanders , I M J G , Sidorov , I A , Mohammed , O A , Savage , E , Timms , A R , Freeman , J , Smits , W K , Wilcox , M H , Baines , S D , Monchi , M (ed.) , Samarkos , M (ed.) & Di Bella , S (ed.) 2023 , ' Non-Toxigenic Clostridioides difficile Strain E4 (NTCD-E4) Prevents Establishment of Primary C. difficile Infection by Epidemic PCR Ribotype 027 in an In Vitro Human Gut Model ' , Antibiotics , vol. 12 , no. 3 , 435 , pp. 1-15 . https://doi.org/10.3390/antibiotics12030435 | |
dc.identifier.issn | 2079-6382 | |
dc.identifier.other | Jisc: 939483 | |
dc.identifier.other | Jisc: 939483 | |
dc.identifier.other | publisher-id: antibiotics-12-00435 | |
dc.identifier.other | ORCID: /0000-0001-7439-1073/work/130605626 | |
dc.identifier.uri | http://hdl.handle.net/2299/26114 | |
dc.description | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |
dc.description.abstract | Clostridioides difficile infection (CDI) remains a significant healthcare burden. Non-toxigenic C. difficile (NTCD) strains have shown a benefit in preventing porcine enteritis and in human recurrent CDI. In this study, we evaluated the efficacy of metronidazole-resistant NTCD-E4 in preventing CDI facilitated by a range of antimicrobials in an in vitro human gut model. NTCD-E4 spores (at a dose of 107) were instilled 7 days before a clinical ribotype (RT) 027 (at the same dose) strain (210). In separate experiments, four different antimicrobials were used to perturb gut microbiotas; bacterial populations and cytotoxin production were determined using viable counting and Vero cell cytotoxicity, respectively. RT027 and NTCD-E4 proliferated in the in vitro model when inoculated singly, with RT027 demonstrating high-level cytotoxin (3-5-log10-relative units) production. In experiments where the gut model was pre-inoculated with NTCD-E4, RT027 was remained quiescent and failed to produce cytotoxins. NTCD-E4 showed mutations in hsmA and a gene homologous to CD196-1331, previously linked to medium-dependent metronidazole resistance, but lacked other metronidazole resistance determinants. This study showed that RT027 was unable to elicit simulated infection in the presence of NTCD-E4 following stimulation by four different antimicrobials. These data complement animal and clinical studies in suggesting NTCD offer prophylactic potential in the management of human CDI. | en |
dc.format.extent | 15 | |
dc.format.extent | 1231571 | |
dc.language.iso | eng | |
dc.relation.ispartof | Antibiotics | |
dc.subject | Article | |
dc.subject | Clostridioides difficile | |
dc.subject | RT027 | |
dc.subject | non-toxigenic | |
dc.subject | antibiotics | |
dc.subject | resistance | |
dc.subject | gut model | |
dc.subject | colonisation | |
dc.subject | infection | |
dc.subject | Microbiology (medical) | |
dc.subject | Infectious Diseases | |
dc.subject | Pharmacology (medical) | |
dc.subject | Biochemistry | |
dc.subject | Pharmacology, Toxicology and Pharmaceutics(all) | |
dc.subject | Microbiology | |
dc.title | Non-Toxigenic Clostridioides difficile Strain E4 (NTCD-E4) Prevents Establishment of Primary C. difficile Infection by Epidemic PCR Ribotype 027 in an In Vitro Human Gut Model | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Microbiology and Biotechnology | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85151742722&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.3390/antibiotics12030435 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |