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dc.contributor.authorSchilstra, M.
dc.contributor.authorRappaport, F.
dc.contributor.authorNugent, J.
dc.contributor.authorBarnett, C.J.
dc.contributor.authorBarber, J.
dc.contributor.authorKlug, D.R.
dc.identifier.citationSchilstra , M , Rappaport , F , Nugent , J , Barnett , C J , Barber , J & Klug , D R 1998 , ' Proton/Hydrogen Transfer Affects the S-State-Dependent Microsecond Phases of P680+ Reduction during Water Splitting ' , Biochemistry , vol. 37 , no. 11 , pp. 3974-3981 .
dc.identifier.otherPURE: 85304
dc.identifier.otherPURE UUID: 2c63b2b9-c54c-4248-824d-bb6e1281c0e2
dc.identifier.otherdspace: 2299/2645
dc.identifier.otherScopus: 0032539967
dc.descriptionOriginal article can be found at: Copyright American Chemical Society DOI: 10.1021/bi9713815 [Full text of this article is not available in the UHRA]
dc.description.abstractTo investigate a possible coupling between P680+ reduction and hydrogen transfer, we studied the effects of H2O/D2O exchange on the P680+ reduction kinetics in the nano- and microsecond domains. We concentrated on studying the period-4 oscillatory (i.e., S-state-related) part of the reduction kinetics, by analyzing the differences between the P680+ reduction curves, rather than the full kinetics. Earlier observations that P680+ reduction kinetics have microsecond components were confirmed: the longest observable lifetime whose amplitude showed period-4 oscillations was 30 μs. We found that solvent isotope exchange left the nanosecond phases of the P680+ reduction unaltered. However, a significant effect on the oscillatory microsecond components was observed. We propose that, at least in the S0/S1 and S3/S0 transitions, hydrogen (proton) transfer provides an additional decrease in the free energy of the YZ+P680 state with respect to the YZP680+ state. This implies that relaxation of the state YZ+P680 is required for complete reduction of P680+ and for efficient water splitting. The kinetics of the P680+ reduction suggest that it is intraprotein proton/hydrogen rearrangement/transfer, rather than proton release to the bulk, which is occurring on the 1−30 μs time scale.en
dc.titleProton/Hydrogen Transfer Affects the S-State-Dependent Microsecond Phases of P680+ Reduction during Water Splittingen
dc.contributor.institutionSchool of Computer Science
dc.contributor.institutionSchool of Creative Arts
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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