dc.contributor.author | Schilstra, M. | |
dc.contributor.author | Rappaport, F. | |
dc.contributor.author | Nugent, J. | |
dc.contributor.author | Barnett, C.J. | |
dc.contributor.author | Barber, J. | |
dc.contributor.author | Klug, D.R. | |
dc.date.accessioned | 2008-11-26T15:20:35Z | |
dc.date.available | 2008-11-26T15:20:35Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | Schilstra , M , Rappaport , F , Nugent , J , Barnett , C J , Barber , J & Klug , D R 1998 , ' Proton/Hydrogen Transfer Affects the S-State-Dependent Microsecond Phases of P680+ Reduction during Water Splitting ' , Biochemistry , vol. 37 , no. 11 , pp. 3974-3981 . https://doi.org/10.1021/bi9713815 | |
dc.identifier.issn | 0006-2960 | |
dc.identifier.other | PURE: 85304 | |
dc.identifier.other | PURE UUID: 2c63b2b9-c54c-4248-824d-bb6e1281c0e2 | |
dc.identifier.other | dspace: 2299/2645 | |
dc.identifier.other | Scopus: 0032539967 | |
dc.identifier.uri | http://hdl.handle.net/2299/2645 | |
dc.description | Original article can be found at: http://pubs.acs.org/journals/bichaw/index.html Copyright American Chemical Society DOI: 10.1021/bi9713815 [Full text of this article is not available in the UHRA] | |
dc.description.abstract | To investigate a possible coupling between P680+ reduction and hydrogen transfer, we studied the effects of H2O/D2O exchange on the P680+ reduction kinetics in the nano- and microsecond domains. We concentrated on studying the period-4 oscillatory (i.e., S-state-related) part of the reduction kinetics, by analyzing the differences between the P680+ reduction curves, rather than the full kinetics. Earlier observations that P680+ reduction kinetics have microsecond components were confirmed: the longest observable lifetime whose amplitude showed period-4 oscillations was 30 μs. We found that solvent isotope exchange left the nanosecond phases of the P680+ reduction unaltered. However, a significant effect on the oscillatory microsecond components was observed. We propose that, at least in the S0/S1 and S3/S0 transitions, hydrogen (proton) transfer provides an additional decrease in the free energy of the YZ+P680 state with respect to the YZP680+ state. This implies that relaxation of the state YZ+P680 is required for complete reduction of P680+ and for efficient water splitting. The kinetics of the P680+ reduction suggest that it is intraprotein proton/hydrogen rearrangement/transfer, rather than proton release to the bulk, which is occurring on the 1−30 μs time scale. | en |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemistry | |
dc.title | Proton/Hydrogen Transfer Affects the S-State-Dependent Microsecond Phases of P680+ Reduction during Water Splitting | en |
dc.contributor.institution | School of Computer Science | |
dc.contributor.institution | School of Creative Arts | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | https://doi.org/10.1021/bi9713815 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |