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dc.contributor.authorMahoney, M.G.
dc.contributor.authorSlakey, L. L.
dc.contributor.authorBenham, C.D.
dc.contributor.authorGross, D. J.
dc.date.accessioned2007-07-25T15:18:52Z
dc.date.available2007-07-25T15:18:52Z
dc.date.issued1998
dc.identifier.citationMahoney , M G , Slakey , L L , Benham , C D & Gross , D J 1998 , ' Time course of the initial [Ca2+]i response to extracellular ATP in smooth muscle depends on [Ca2+]e and ATP concentration ' , Biophysical Journal , vol. 75 , no. 4 , pp. 2050-2058 . https://doi.org/10.1016/S0006-3495(98)77647-7
dc.identifier.issn0006-3495
dc.identifier.otherPURE: 123886
dc.identifier.otherPURE UUID: fac2ff37-efeb-4d7b-9d58-1eb324eca660
dc.identifier.otherdspace: 2299/275
dc.identifier.otherScopus: 0031718946
dc.identifier.urihttp://hdl.handle.net/2299/275
dc.descriptionThe final copyedited version of the paper may be found at www.biophysj.org. Copyright the Biophysical Society. DOI : 0006-3495/98/10/2050/09
dc.description.abstractIn response to extracellular application of 50 mM ATP, all individual porcine aortic smooth muscle cells respond with rapid rises from basal [Ca21]i to peak [Ca21]i within 5 s. The time from stimulus to the peak of the [Ca21]i response increases with decreasing concentration of ATP. At ATP concentrations of 0.5 mM and below, the time to the [Ca21]i peak varies more significantly from cell to cell than at higher concentrations, and each cell shows complicated initiation and decay kinetics. For any individual cell, the lag phase before a response decreases with increasing concentration of ATP. An increase in lag time with decreasing ATP concentration is also observed in the absence of extracellular Ca21, but the lag phase is more pronounced, especially at concentrations of ATP below 0.5 mM. Whole-cell patch-clamp electrophysiology shows that in porcine aortic smooth muscle cells, ATP stimulates an inward current carried mainly by Cl2 ion efflux with a time course similar to the [Ca21]i changes and no detectable current from an ATP-gated cation channel. A simple signal cascade initiation kinetics model, starting with nucleotide receptor activation leading to IP3-mediated Ca21 release from IP3-sensitive internal stores, fits the data and suggests that the kinetics of the Ca21 response are dominated by upstream signal cascade components.en
dc.language.isoeng
dc.relation.ispartofBiophysical Journal
dc.rightsOpen
dc.titleTime course of the initial [Ca2+]i response to extracellular ATP in smooth muscle depends on [Ca2+]e and ATP concentrationen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dcterms.dateAccepted1998
rioxxterms.versionofrecordhttps://doi.org/10.1016/S0006-3495(98)77647-7
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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