dc.contributor.author | Schilstra, M. | |
dc.contributor.author | Martin, S.R. | |
dc.contributor.author | Bayley, P.M. | |
dc.date.accessioned | 2009-09-29T14:38:26Z | |
dc.date.available | 2009-09-29T14:38:26Z | |
dc.date.issued | 1987 | |
dc.identifier.citation | Schilstra , M , Martin , S R & Bayley , P M 1987 , ' On the relationship between nucleotide hydrolysis and microtubule assembly : Studies with a GTP-regenerating system ' , Biochemical and Biophysical Research Communications , vol. 147 , no. 2 , pp. 588-595 . https://doi.org/10.1016/0006-291X(87)90971-5 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.other | PURE: 98366 | |
dc.identifier.other | PURE UUID: 01640559-e914-4fad-a0f9-e858fb029aca | |
dc.identifier.other | dspace: 2299/3899 | |
dc.identifier.other | Scopus: 0023656098 | |
dc.identifier.uri | http://hdl.handle.net/2299/3899 | |
dc.description | Original article can be found at: http://www.sciencedirect.com/science/journal/0006291X Copyright Elsevier Inc. [Full text of this article is not available in the UHRA] | |
dc.description.abstract | The assembly of pure tubulin dimer has been studied in two buffer systems (containing low and high glycerol/Mg), using a regeneration system protocol to assess the amount of GDP-tubulin in the assembling polymer. For both assembly systems studied, the GDP content is effectively stoichiometric with tubulin throughout assembly. This indicates a high degree of coupling between assembly and GTP-hydrolysis, giving a hydrolysis rate at least 10-fold faster than previously deduced. The steady state GTP hydrolysis rate is quantitatively consistent with this finding. We conclude that the extent of any GTP-tubulin cap is below the detectable limit, both during elongation and at steady state. MT-protein; microtubule protein; MAPs; microtubule associated proteins; PC-tubulin; tubulin dimer from phosphocellulose chromatography; GTP- and GDP-tubulin denotes the nucleotide at the exchangeable nucleotide binding site (E-site)RS; enzymic GTP-regenerating system. | en |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | |
dc.title | On the relationship between nucleotide hydrolysis and microtubule assembly : Studies with a GTP-regenerating system | en |
dc.contributor.institution | School of Computer Science | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | https://doi.org/10.1016/0006-291X(87)90971-5 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |