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dc.contributor.authorHoskins, C.
dc.contributor.authorOuaissi, M.
dc.contributor.authorLima, S.C.
dc.contributor.authorCheng, W.P.
dc.contributor.authorLoureirio, I.
dc.contributor.authorMas, E.
dc.contributor.authorLombardo, D.
dc.contributor.authorCordeiro-da-Silva, A.
dc.date.accessioned2011-01-31T11:21:10Z
dc.date.available2011-01-31T11:21:10Z
dc.date.issued2010
dc.identifier.citationHoskins , C , Ouaissi , M , Lima , S C , Cheng , W P , Loureirio , I , Mas , E , Lombardo , D & Cordeiro-da-Silva , A 2010 , ' In Vitro and In Vivo Anticancer Activity of a Novel Nano-sized Formulation Based on Self-assembling Polymers Against Pancreatic Cancer ' , Pharmaceutical Research , vol. 27 , no. 12 , pp. 2694-2703 . https://doi.org/10.1007/s11095-010-0268-6
dc.identifier.issn0724-8741
dc.identifier.otherdspace: 2299/5251
dc.identifier.urihttp://hdl.handle.net/2299/5251
dc.descriptionThe original publication is available at www.springerlink.com. Copyright Springer
dc.description.abstractPurpose: To evaluate the in vitro and in vivo pancreatic anticancer activity of a nano-sized formulation based on novel polyallylamine grafted with 5% mole cholesteryl pendant groups (CH5-PAA). Methods: Insoluble novel anticancer drug, Bisnaphthalimidopropyldiaaminooctane (BNIPDaoct), was loaded into CH5-PAA polymeric self-assemblies by probe sonication. Hydrodynamic diameters and polydispersity index measurements were determined by photon correlation spectroscopy. The in vitro cytotoxicity evaluation of the formulation was carried out by the sulforhodamine B dye assay with human pancreatic adenocarcinoma BxPC-3 cells, while for the in vivo study, Xenograff mice were used. In vitro apoptotic cell death from the drug formulation was confirmed by flow cytometric analysis. Results: The aqueous polymer-drug formulation had a mean hydrodynamic size of 183 nm. The drug aqueous solubility was increased from negligible concentration to 0.3 mg mL−1. CH5-PAA polymer alone did not exhibit cytotoxicity, but the new polymer-drug formulation showed potent in vitro and in vivo anticancer activity. The mode of cell death in the in vitro study was confirmed to be apoptotic. The in vivo results revealed that the CH5-PAA alone did not have any anti-proliferative effect, but the CH5-PAA-drug formulation exhibited similar tumour reduction efficacy as the commercial drug, gemcitabine. Conclusions: The proposed formulation shows potential as pancreatic cancer therapeutics.en
dc.format.extent7098
dc.format.extent11087
dc.format.extent17420
dc.format.extent17307
dc.format.extent17283
dc.format.extent20542
dc.format.extent4494
dc.format.extent4638
dc.format.extent199606
dc.language.isoeng
dc.relation.ispartofPharmaceutical Research
dc.subjectPancreatic cancer
dc.subjectself-assembling polymers
dc.subjectamphiphilic polyallylamine
dc.subjectnanoparticles
dc.subjectBisnaphthalimidopropyl-diaminooctane
dc.subjectapoptosis
dc.subjectBxPC-3 cells
dc.titleIn Vitro and In Vivo Anticancer Activity of a Novel Nano-sized Formulation Based on Self-assembling Polymers Against Pancreatic Canceren
dc.contributor.institutionDepartment of Pharmacy
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1007/s11095-010-0268-6
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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