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dc.contributor.authorSmith, E.J.
dc.contributor.authorHoffman, R.
dc.date.accessioned2011-01-31T14:58:41Z
dc.date.available2011-01-31T14:58:41Z
dc.date.issued2005
dc.identifier.citationSmith , E J & Hoffman , R 2005 , ' Multiple fragments related to angiostatin and endostatin in fluid from venous leg ulcers ' , Wound Repair and Regeneration , vol. 13 , no. 2 , pp. 148-157 . https://doi.org/10.1111/j.1067-1927.2005.130205.x
dc.identifier.issn1067-1927
dc.identifier.otherdspace: 2299/5253
dc.identifier.urihttp://hdl.handle.net/2299/5253
dc.descriptionThe definitive version is available at www.blackwell-synergy.com Copyright Blackwell Publishing [Full text of this article is not available in the UHRA]
dc.description.abstractTo investigate whether compromised angiogenesis could contribute to the impaired healing of venous leg ulcers, we have analyzed fluids from venous leg ulcers for the presence of the angiogenesis inhibitors angiostatin and endostatin. Multiple fragments related to angiostatin were detected by Western blot analysis. One angiostatin fragment was identified by mass spectrometry as plasminogen kringle domains 1–3 containing amino acids 82–343 of plasminogen, and a fraction containing this fragment inhibited tubule formation of human umbilical vein endothelial cells in a Matrigel assay. The leg ulcer fluids also contained endogenous endostatin (20 kDa) as well as higher molecular weight endostatin-related proteins. The concentrations of endostatin in the wound fluids, which ranged from 12.8 to 65.5 ng/ml, were higher than the concentration in human serum (7.7 ng/ml). Most of the endostatin in leg ulcer fluid appeared to be bound to the proteoglycan glypican-1. These data suggest that anti-angiogenic activity is present at the site of venous leg ulcers, and at least in the case of angiostatin, is biologically active.en
dc.language.isoeng
dc.relation.ispartofWound Repair and Regeneration
dc.titleMultiple fragments related to angiostatin and endostatin in fluid from venous leg ulcersen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1111/j.1067-1927.2005.130205.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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