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Browsing University of Hertfordshire by Author "Wood, K.R."
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Improved isolation and culture methods for cucumber mesophyll protoplasts
Coutts, Robert H.A.; Wood, K.R. (1977-05-01)Improved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods ... -
The infection of cucumber mesophyll protoplasts with tobacco mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1976)Protoplasts from the first leaf mesophyll of cucumber plants were isolated by an 18 hour combined petinase/cellulase treatment. Conditions favouring the infection of these protoplasts with tobacco mosaic virus (TMV), and ... -
Inoculation of leaf mesophyll protoplasts from a resistant and a susceptible cucumber cultivar with cucumber mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1977-01) -
Investigations on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1976)Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts ... -
The isolation and culture of cucumber mesophyll protoplasts
Coutts, Robert H.A.; Wood, K.R. (1975-03-01)Large numbers of viable protoplasts were obtained from the first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China. Cell wall regeneration was observed in liquid culture within 3 days of isolation, ... -
Preliminary studies on the RNA components of three strains of cucumber mosaic virus
Wood, K.R.; Coutts, Robert H.A. (1975-11)In investigations of the causal relationships between disease symptoms and virus infection of plants, three strains of cucumber mosaic virus (viz. N, Price's No. 6 and W) which caused different symptoms in several plant ... -
Ribosomal RNA metabolism in cucumber leaf mesophyll protoplasts
Coutts, Robert H.A.; Barnett, A.; Wood, K.R. (1975-07-01)Aspects of the metabolism of RNA have been studied in enzymatically isolated protoplasts from cotyledon and first leaf mesophyll tissue of two cultivars of cucumber. The first leaf mesophyll protoplasts incorporated ( ...