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Alterations in the soluble protein patterns of tobacco and cowpea leaves following inoculation with tobacco necrosis virus
(1978-06-01)
Changes in the soluble protein composition of both tobacco and cowpea leaves following inoculation with tobacco necrosis virus (TNV R/1 : 1.5/19 : S/S : S/Fu [1]) have been investigated by the techniques of disc-electrophoresis. ...
Suppression of virus induced local lesions in plasmolysed leaf tissue
(1978-05-01)
It has been demonstrated previously that protoplasts derived from leaves which normally respond in vivo to virus inoculation, with the formation of necrotic local lesions show no such response when inoculated in vitro [1]. ...
Improved isolation and culture methods for cucumber mesophyll protoplasts
(1977-05-01)
Improved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods ...
Investigations on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus
(1976)
Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts ...
The infection of cucumber mesophyll protoplasts with tobacco mosaic virus
(1976)
Protoplasts from the first leaf mesophyll of cucumber plants were isolated by an 18 hour combined petinase/cellulase treatment. Conditions favouring the infection of these protoplasts with tobacco mosaic virus (TMV), and ...
Preliminary studies on the RNA components of three strains of cucumber mosaic virus
(1975-11)
In investigations of the causal relationships between disease symptoms and virus infection of plants, three strains of cucumber mosaic virus (viz. N, Price's No. 6 and W) which caused different symptoms in several plant ...
Ribosomal RNA metabolism in cucumber leaf mesophyll protoplasts
(1975-07-01)
Aspects of the metabolism of RNA have been studied in enzymatically isolated protoplasts from cotyledon and first leaf mesophyll tissue of two cultivars of cucumber. The first leaf mesophyll protoplasts incorporated ( ...
The isolation and culture of cucumber mesophyll protoplasts
(1975-03-01)
Large numbers of viable protoplasts were obtained from the first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China. Cell wall regeneration was observed in liquid culture within 3 days of isolation, ...
Early stages in cell wall regeneration of tobacco mesophyll protoplasts : An electrophoretic study
(1975-02-01)
The electrophoretic mobilities of isolated mesophyll protoplasts of Nicotiana tabacum var. Xanthi n.c. were measured using a whole cell electrophoresis apparatus. Changes in mobility were recorded at times during aseptic ...