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Amanicadol, a pimarane-type diterpene from Phlomis amanica Vierch
Fractionation of the methanol extract of Phlomis amanica resulted in the isolation of a new pimarane type diterpene, amanicadol (1), together with the known glycosides lamiide, verbascoside (= acteoside), syringaresinol-4-O-β- ...
Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells
We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its ...
Synthesis of DNA-directed pyrrolidinyl and piperidinyl confined alkylating chloroalkylaminoanthraquinones : potential for development of tumor-selective N-oxides
A novel series of 1,4-disubstituted chloroethylaminoanthraquinones, containing alkylating chloroethylamino functionalities as part of a rigid piperidinyl or pyrrolidinyl ring-system, have been prepared. The target compounds ...
Site-specific PEGylation of native disulfide bonds in therapeutic proteins
Native disulfide bonds in therapeutic proteins are crucial for tertiary structure and biological activity and are therefore considered unsuitable for chemical modification. We show that native disulfides in human interferon ...
PEGylation of native disulfide bonds in proteins
PEGylation has turned proteins into important new biopharmaceuticals. The fundamental problems with the existing approaches to PEGylation are inefficient conjugation and the formation of heterogeneous mixtures. This is ...