Browsing Research publications by Author "Asher, M. J. C."
Now showing items 1-15 of 15
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The action of three Beet yellows virus resistance QTLs depends on alleles at a novel genetic locus that controls symptom development
Grimmer, M. K.; Bean, K. M. R.; Qi, Aiming; Stevens, M.; Asher, M. J. C. (2008-08)Beet yellows virus (BYV) can dramatically reduce yield from sugar beet crops. A BYV resistant plant from a Beta germplasm accession was crossed with a sugar beet plant to generate the segregating population BYV1. This ... -
Barley elicits a similar early basal defence response during host and non-host interactions with Polymyxa root parasites
McGrann, G.; Townsend, B.; Antoniw, J.F.; Asher, M. J. C.; Mutasa-Gottgens, Euphemia (2009-01)Plant viruses transmitted by the obligate root-infecting plasmodiophorid parasites Polymyxa graminis and Polymyxa betae cause devastating yield losses to cereal and sugar beet crops worldwide. Barley is a non-host for P. ... -
Characterization of Polymyxa species by restriction analysis of PCR-amplified ribosomal DNA
Ward, Elaine; Adams, Michael J.; Mutasa, E. S.; Collier, C. R.; Asher, M. J. C. (1994-10)A molecular method is described to aid identification of the obligate parasite Polymyxa and discriminate between species (P. betae and P. graminis) and isolates. DNA was extracted from zoospores, resting spores and roots ... -
Development and Application of Molecular Methods for the Study of Polymyxa betae
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Williams, G.; Ward, E.; Adams, M. J.; Asher, M. J. C. (American Society of Sugar Beet Technologists, 1996)A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. ... -
The Development of a quantitative ELISA for the detection of Polymyxa betae
Kingsnorth, Crawford S.; Mutasa-Gottgens, Euphemia; Asher, M. J. C. (American Society of Sugar Beet Technologists, 2003) -
The development of a recombinant antibody to Polymyxa betae and its use in resistance screening
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Kingsnorth, Crawford S.; Halsey, K.; Asher, M. J. C. (American Society of Sugar Beet Technologists, 2000)Antibodies to the obligate sugar-beet root parasite Polymyxa betae have been produced using a novel approach that selects for fungal gene sequences encoding proteins that are highly expressed in planta during infection. ... -
The development of Diagnostics for Evaluating Beta Germplasm
Thomas, T.H.; Asher, M. J. C.; Smith, H.G.; Clarke, N.A.; Mutasa-Gottgens, Euphemia; Thompson, J.R. (1993)The development of diagnostics for evaluating Beta germ plasm should lead to the more informative cataloguing and rapid exploitation of Beta gene banks. At Broom's Barn Experimental Station, resistance or tolerance to aphid ... -
Development of PCR for the detection of Polymyxa-betae in sugar beet roots and its application in field studies
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Adams, Michael J.; Ward, Elaine; Asher, M. J. C. (1995-11)A previously cloned 1.8 kb PCR Polymyxa betae DNA fragment has been fully sequenced and used to design PCR primers for the specific detection of P. betae in sugar beet seedling roots. The primers did not amplify sequences ... -
Isolation and characterization of genes induced during non-host resistance to Polymyxa spp
Kingsnorth, Crawford S.; Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Asher, M. J. C. (American Society of Sugar Beet Technologists, 2003) -
Molecular Biology : How can it help the sugar beet industry?
Asher, M. J. C.; Mutasa-Gottgens, Euphemia; Stevens, M.; Van Roggen, P. M.; Scott, R. (1996) -
Rhizomania : The role of vector and virus resistnace
Asher, M. J. C.; Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M. (1997) -
Screening for resistance to Polymyxa betae : the development of a recombinant antiserum
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Kingsnorth, C.S.; Halsey, K.; Asher, M. J. C. (2000) -
The selection and characterisation of resistance to Polymyxa betae, vector of Beet necrotic yellow vein virus, derived from wild sea beet.
Asher, M. J. C.; Grimmer , M. K.; Mutasa-Gottgens, Euphemia (2009)The plasmodiophoromycete Polymyxa betae is an obligate root parasite that transmits Beet necrotic yellow vein virus (BNYVV), the cause of sugar beet rhizomania disease. Currently, control of this disease is achieved through ... -
A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots
Mutasa-Gottgens, Euphemia; Ward, Elaine; Adams, M. J.; Collier, C. R.; Chwarszynska, D. M.; Asher, M. J. C. (1993-11)A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment ... -
Single-tube, nested PCR for the diagnosis of Polymyxa betae infection in sugar beet roots and colorimetric analysis of amplified products
Mutasa-Gottgens, Euphemia; Chwarszczynska, D.M.; Asher, M. J. C. (1996-05)Nested primers for the specific amplification of DNA sequences from the obligate parasitic root-infecting fungus Polymyxa betae in a single-tube reaction are described. The choice of primers, DNA purity, and relative ...