Browsing Research publications by Author "Offei, S.K."
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Location of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAs
Offei, S.K.; Coutts, Robert H.A. (1996-01)Northern blot analysis of double-stranded (ds) RNA from bean-leaf tissue infected with tobacco necrosis virus strain D (TNV-D) detected the 4 kb genomic RNA and two subgenomic RNAs of about 1.5 kb and 1.2 kb; RNA extracted ... -
Molecular variation of Potato yellow vein virus isolates
Offei, S.K.; Arciniegas, N.; Guzmán, M.; Müller, G.; Salazar, L.F.; Coutts, Robert H.A. (2004-04-01)To evaluate the variation of Potato yellow vein virus from potato fields, 12 isolates were collected from Colombia and one was collected from Peru. Double-stranded RNA was extracted from the plants and used as a template ... -
Subcellular location and expression of tobacco Necrosis necrovirus p7a protein
Drouzas, A.D.; Offei, S.K.; Coutts, Robert H.A. (1996-06)A putative movement protein (p7a) of tobacco necrosis Necrovirus, strain D (TNV-D), produced in Escherichia coli using an expression vector, was used to raise an antiserum. Immunoblot analysis using this antiserum showed ... -
The tobacco necrosis virus p7a protein is a nucleic acid-binding protein
Offei, S.K.; Coffin, R.S.; Coutts, Robert H.A. (1995-06)The two centrally located open reading frames (ORFs) of necroviruses may, by analogy with the similarly located and related ORFs of carmoviruses, be expected to have a function in virus movement in plants. In the case of ...