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Improved isolation and culture methods for cucumber mesophyll protoplasts
(1977-05-01)
Improved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods ...
Investigations on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus
(1976)
Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts ...
The infection of cucumber mesophyll protoplasts with tobacco mosaic virus
(1976)
Protoplasts from the first leaf mesophyll of cucumber plants were isolated by an 18 hour combined petinase/cellulase treatment. Conditions favouring the infection of these protoplasts with tobacco mosaic virus (TMV), and ...
Preliminary studies on the RNA components of three strains of cucumber mosaic virus
(1975-11)
In investigations of the causal relationships between disease symptoms and virus infection of plants, three strains of cucumber mosaic virus (viz. N, Price's No. 6 and W) which caused different symptoms in several plant ...
Ribosomal RNA metabolism in cucumber leaf mesophyll protoplasts
(1975-07-01)
Aspects of the metabolism of RNA have been studied in enzymatically isolated protoplasts from cotyledon and first leaf mesophyll tissue of two cultivars of cucumber. The first leaf mesophyll protoplasts incorporated ( ...
The isolation and culture of cucumber mesophyll protoplasts
(1975-03-01)
Large numbers of viable protoplasts were obtained from the first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China. Cell wall regeneration was observed in liquid culture within 3 days of isolation, ...