PCR mutation detection protocols
This is the second edition of a text that was followed by the huge growth in DNA sequence information, the availability of bioinformatics and mutation databases, and the resources to detect and confirm mutations. This all secured the place of molecular diagnostics in medicine as well as mutation detection by the polymerase chain reaction (PCR). In this text researchers of the field renew this subject to encompass contemporary information and key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The book further explores core topics such as Southern blotting, accurate diagnostics with high throughput, and microarray systems. The book is composed in the popular series format of Methods in Molecular Biology. Its chapters include brief introductions of the topics, lists of the materials and reagents that are essential, list-form easily to follow laboratory protocols, and notes which provide often hidden information that is necessary to implement the method successfully. This text serves to help postgraduate scientists, researchers, and clinicians who are already occupied in the field as well as supplying a solid first step to those who are new to the notion of employing this method in the laboratory.