dc.contributor.author | Sharp, A.J. | |
dc.contributor.author | Walker, John | |
dc.date.accessioned | 2011-08-22T09:01:05Z | |
dc.date.available | 2011-08-22T09:01:05Z | |
dc.date.issued | 1993 | |
dc.identifier.citation | Sharp , A J & Walker , J 1993 , ' Mung-bean nuclease 1 (EC 3.1.30.1) ' , Methods in Molecular Biology , vol. 16 , pp. 253-261 . https://doi.org/10.1385/0-89603-234-5:253 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | http://hdl.handle.net/2299/6325 | |
dc.description | The original publication is available at www.springerlink.com Copyright Humana Press [Full text of this article is not available in the UHRA] | |
dc.description.abstract | Mung-bean nuclease 1 was first purified by Sung and Laskowski (1) in 1962 from mung-bean sprouts (Phaseolus aureus). It belongs to the class of enzymes EC 3.1.30.1., which has a preference for single-stranded nucleic acid substrates, lacks sugar specificity, and hydrolyzes single-stranded substrates to produce products with 5-phoshoryl and 3-hydroxl termini, ranging fro mono-to, at least, heptanucleotides. Although it shows a preference for single-stranded nucleic acids over double-stranded of 30,000-fold (2), used in high concentrations with extended incubation times, mung-bean nuclease 1 will completely degrade double-stranded DNA (3-5). Mung-bean nuclease 1 is also reported to show a separate 3-(1)-monophosphatase activity (6). Mung-bean nuclease 1 is a zinc metalloenzyme that requires Zn2+ and a reducing agent, such as cysteine, for both activity and stability. | en |
dc.language.iso | eng | |
dc.relation.ispartof | Methods in Molecular Biology | |
dc.title | Mung-bean nuclease 1 (EC 3.1.30.1) | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1385/0-89603-234-5:253 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |