An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles.
Kaye, Paul H.
Clark, J M
Withers, P B
We describe the construction of a bio-aerosol monitor designed to capture and record intrinsic fluorescence spectra from individual aerosol particles carried in a sample airflow and to simultaneously capture data relating to the spatial distribution of elastically scattered light from each particle. The spectral fluorescence data recorded by this PFAS (Particle Fluorescence and Shape) monitor contains information relating to the particle material content and specifically to possible biological fluorophores. The spatial scattering data from PFAS yields information relating to particle size and shape. The combination of these data can provide a means of aiding the discrimination of bio-aerosols from background or interferent aerosol particles which may have similar fluorescence properties but exhibit shapes and/or sizes not normally associated with biological particles. The radiation used both to excite particle fluorescence and generate the necessary spatially scattered light flux is provided by a novel compact UV fiber laser operating at 266nm wavelength. Particles drawn from the ambient environment traverse the laser beam in single file. Intrinsic particle fluorescence in the range 300-570nm is collected via an ellipsoidal concentrator into a concave grating spectrometer, the spectral data being recorded using a 16-anode linear array photomultiplier detector. Simultaneously, the spatial radiation pattern scattered by the particle over 5degrees-30degrees scattering angle and 360degrees of azimuth is recorded using a custom designed 31-pixel radial hybrid photodiode array. Data from up to similar to5,000 particles per second may be acquired for analysis, usually performed by artificial neural network classification.
Published inOPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE
RelationsSchool of Physics, Astronomy and Mathematics
MetadataShow full item record
Showing items related by title, author, creator and subject.
First correlated measurements of the shape and scattering properties of cloud particles using the new Particle Habit Imaging and Polar Scattering (PHIPS) probe Abdelmonem, A.; Schnaiter, M.; Amsler, P.; Hesse, Evelyn; Meyer, J.; Leisner, T. (2011)Studying the radiative impact of cirrus clouds requires the knowledge of thelink between their microphysics and the single scattering properties of thecloud particles. Usually, this link is created by modeling the ...
Angularly resolved elastic scattering from airborne particles - Potential for characterizing, classifying, and identifying individual aerosol particles Kaye, Paul H.; Aptowicz, Kevin; Chang, Richard K.; Foot, Virginia; Videen, Gorden (Human Press / Springer, 2007)Analysing the light scattering properties of individual airborne particles has become a powerful tool by which they may be characterized, classified, and in some cases, identified. The approach offers a non-invasive, ...
Single-Particle Raman Spectrometer (SPaRS) for the non-destructive physico-chemical characterisation of aerosols Greenaway, R.; Dalley, J.E.J.; Ulanowski, Z.; Hesse, E.; Kaye, Paul H. (2004)This project seeks to develop a prototype systems for the analysis of single levitated micrometre-sized particles captured from an ambient aerosol. The systems is designed to use Raman scattering to establish chemical ...