Show simple item record

dc.contributor.authorStair, Jacqueline L.
dc.contributor.authorWhite, Brianna R.
dc.contributor.authorRowland, Adam
dc.contributor.authorHolcombe, James A.
dc.date.accessioned2012-01-17T18:01:11Z
dc.date.available2012-01-17T18:01:11Z
dc.date.issued2006-11-13
dc.identifier.citationStair , J L , White , B R , Rowland , A & Holcombe , J A 2006 , ' Quantitative determination of single-bead metal content from a peptide combinatorial library ' , Journal of Combinatorial Chemistry , vol. 8 , no. 6 , pp. 929-934 . https://doi.org/10.1021/cc060100m
dc.identifier.issn1520-4766
dc.identifier.otherPURE: 526947
dc.identifier.otherPURE UUID: c490ad3f-0685-474d-9307-428e026c92ae
dc.identifier.otherWOS: 000241942100019
dc.identifier.otherScopus: 33845269947
dc.identifier.urihttp://hdl.handle.net/2299/7677
dc.description.abstractAn electrothermal vaporizer inductively coupled plasma mass spectrometer (ETV-ICPMS) was used to quantitatively screen metals bound to single polystyrene (TentaGel) beads with immobilized oligopeptides. Tests were performed using ETV-ICPMS to screen a series of identical beads as well as a series of combinatorial library beads exposed to a multimetal solution composed of Mg2+, Mn2+, Ni2+, Cu2+, Cd2+, Eu2+, and Pb2+. The residual metal content remaining bound to the beads after acid extractions was also analyzed by solid sampling of the entire bead using oxygen ashing in the ETV. Nine beads (80 mesh, 0.25 mmol g(-1) nominal capacity) containing covalently attached polyaspartic acid (PLAsp; n = 20) showed metal extract concentrations in the range of 4-130 ng mL(-1). After normalizing by bead volume, the precision of capacity measurements in a single bead (7-14%) was primarily dictated by analysis error and contributions from bead diameter measurement with negligible contributions, surprisingly, from variations in site density from bead to bead. A sample combinatorial library of the sequence GXXGXXGXXGXX (X = cysteine, aspartic acid, or glutamic acid and G = glycine) (60 mesh, 0.25 mmol g(-1) nominal capacity) was also used to demonstrate the utility of this method. Metal extract concentrations ranged from 1 to 1300 ng mL(-1) with significant concentration variation between beads, indicating the individual selectivity on each bead. For these larger beads, analysis precision (i.e., capacity precision) was further improved to 3-10% due to the overall increase in bead metal content. Through metal extract determinations, ETV-ICPMS was shown to be a viable nondestructive tool for full metal characterization of "hit" sequences belonging to a combinatorial library.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofJournal of Combinatorial Chemistry
dc.subjectSOLID SUPPORTS
dc.subjectFLUORESCENCE MICROSCOPY
dc.subjectDISCOVERY
dc.subjectBINDING
dc.subjectREMEDIATION
dc.subjectCOMPLEXES
dc.subjectCHEMISTRY
dc.subjectCATALYSTS
dc.titleQuantitative determination of single-bead metal content from a peptide combinatorial libraryen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1021/cc060100m
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record