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dc.contributor.authorHirsch, P R
dc.contributor.authorAtkins, S D
dc.contributor.authorMauchline, T H
dc.contributor.authorMorton, C O
dc.contributor.authorDavies, Keith
dc.contributor.authorKerry, B R
dc.date.accessioned2012-02-21T17:54:49Z
dc.date.available2012-02-21T17:54:49Z
dc.date.issued2001
dc.identifier.citationHirsch , P R , Atkins , S D , Mauchline , T H , Morton , C O , Davies , K & Kerry , B R 2001 , ' Methods for studying the nematophagous fungus Verticillium chlamydosporium in the root environment ' Plant and Soil , vol. 232 , no. 1-2 , pp. 21-30 . https://doi.org/10.1023/A:1010373717186
dc.identifier.issn0032-079X
dc.identifier.otherPURE: 590417
dc.identifier.otherPURE UUID: f65a1dc2-01ec-43bb-9c9b-3137a86d3cba
dc.identifier.otherWOS: 000169218200004
dc.identifier.otherScopus: 0034938948
dc.identifier.urihttp://hdl.handle.net/2299/7859
dc.description.abstractIn order to exploit fully the biocontrol potential of the nematophagous fungus Verticillium chlamydosporium, it is important to understand the ecology of the fungus in soil, and interactions with both plant and nematode hosts. Several approaches for studying the fungus in soil and the root environment are compared. These include a semi-selective medium for V. chlamydosporium, PCR primers specific for the fungal beta -tubulin gene, and monoclonal antibodies. In addition to providing a target for species-specific primers, the beta -tubulin gene is implicated in resistance to the fungicides used in the semi-selective medium, and the genetic basis for this is investigated. Culture and PCR-based methods were used to screen for the presence of the fungus in field soils known to have been suppressive to cereal cyst nematode and that contained V. chlamydosporium populations. Monoclonal antibodies specific for either hyphae or conidia of the fungus were obtained, and their application as a tool for visualising the infection process on the root was explored.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofPlant and Soil
dc.subjectVerticillium chlamydosporium
dc.subjectbiocontrol
dc.subjectPCR detection
dc.subjectbeta-tubulin gene
dc.subjectmonoclonal antibodies
dc.subjectimmunofluorescence
dc.subjectCEREAL CYST-NEMATODE
dc.subjectHETERODERA-AVENAE
dc.subjectSOIL
dc.subjectPCR
dc.subjectGODDARD
dc.subjectPLANT
dc.titleMethods for studying the nematophagous fungus Verticillium chlamydosporium in the root environmenten
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1023/A:1010373717186
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstyperestrictedAccess


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