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dc.contributor.authorCheng, Woei Ping
dc.contributor.authorHoskins, Clare
dc.contributor.authorWang, LY
dc.contributor.authorCuschieri, A
dc.date.accessioned2012-03-20T15:00:45Z
dc.date.available2012-03-20T15:00:45Z
dc.date.issued2012
dc.identifier.citationCheng , W P , Hoskins , C , Wang , LY & Cuschieri , A 2012 , ' Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering : which tests and what protocols? ' Nanoscale Research Letters , vol. 7 , 77 . https://doi.org/10.1186/1556-276X-7-77
dc.identifier.issn1556-276X
dc.identifier.otherPURE: 635622
dc.identifier.otherPURE UUID: 7f23977d-25a5-404e-ad37-e54c958741a1
dc.identifier.otherScopus: 84862971823
dc.identifier.urihttp://hdl.handle.net/2299/7993
dc.description.abstractMagnetic nanoparticles [MNPs] made from iron oxides have many applications in biomedicine. Full understanding of the interactions between MNPs and mammalian cells is a critical issue for their applications. In this study, MNPs were coated with poly(ethylenimine) [MNP-PEI] and poly(ethylene glycol) [MNP-PEI-PEG] to provide a subtle difference in their surface charge and their cytotoxicity which were analysed by three standard cell viability assays: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium [MTS], CellTiter-Blue and CellTiter-Glo (Promega, Southampton, UK) in SH-SY5Y and RAW 264.7 cells The data were validated by traditional trypan blue exclusion. In comparison to trypan blue manual counting, the MTS and Titer-Blue assays appeared to have consistently overestimated the viability. The Titer-Glo also experienced a small overestimation. We hypothesise that interactions were occurring between the assay systems and the nanoparticles, resulting in incorrect cell viability evaluation. To further understand the cytotoxic effect of the nanoparticles on these cells, reactive oxygen species production, lipid peroxidation and cell membrane integrity were investigated. After pegylation, the MNP-PEI-PEG possessed a lower positive surface charge and exhibited much improved biocompatibility compared to MNP-PEI, as demonstrated not only by a higher cell viability, but also by a markedly reduced oxidative stress and cell membrane damage. These findings highlight the importance of assay selection and of dissection of different cellular responses in in-vitro characterisation of nanostructures.en
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofNanoscale Research Letters
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.titleDilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering : which tests and what protocols?en
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionModified Release Dosage Forms
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.description.versiontypeFinal Published version
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1186/1556-276X-7-77
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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