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dc.contributor.authorPuinean, Alin M.
dc.contributor.authorFoster, Stephen P.
dc.contributor.authorOliphant, Linda
dc.contributor.authorDenholm, Ian
dc.contributor.authorField, Linda M.
dc.contributor.authorMillar, Neil S.
dc.contributor.authorWilliamson, Martin S.
dc.contributor.authorBass, Chris
dc.date.accessioned2012-04-10T09:58:17Z
dc.date.available2012-04-10T09:58:17Z
dc.date.issued2010-06
dc.identifier.citationPuinean , A M , Foster , S P , Oliphant , L , Denholm , I , Field , L M , Millar , N S , Williamson , M S & Bass , C 2010 , ' Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae ' , PLoS Genetics , vol. 6 , no. 6 , e1000999 . https://doi.org/10.1371/journal.pgen.1000999
dc.identifier.issn1553-7390
dc.identifier.otherPURE: 772775
dc.identifier.otherPURE UUID: cf332816-60bc-40e6-ab94-301e09eac41c
dc.identifier.otherWOS: 000279805200028
dc.identifier.otherScopus: 77954154230
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873699
dc.identifier.urihttp://hdl.handle.net/2299/8237
dc.description.abstractThe aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in M. persicae represent a threat to the long-term efficacy of this chemical class. In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches. Bioassays on a resistant M. persicae clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in M. persicae, revealed constitutive over-expression (22-fold) of a single P450 gene (CYP6CY3); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification. This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest. The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2-16-fold), and artificial feeding assays and in vivo penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance. Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in M. persicae.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofPLoS Genetics
dc.subjectBEMISIA-TABACI HEMIPTERA
dc.subjectPEACH-POTATO APHIDS
dc.subjectCOPY-NUMBER
dc.subjectIDENTIFICATION
dc.subjectALEYRODIDAE
dc.subjectPENETRATION
dc.subjectPOPULATIONS
dc.subjectMECHANISMS
dc.subjectEXPRESSION
dc.subjectDROSOPHILA
dc.titleAmplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicaeen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pgen.1000999
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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