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dc.contributor.authorDavies, Keith
dc.contributor.authorLander, E. B.
dc.date.accessioned2012-04-18T07:57:55Z
dc.date.available2012-04-18T07:57:55Z
dc.date.issued1992-07
dc.identifier.citationDavies , K & Lander , E B 1992 , ' Immunological differentiation of root-knot nematodes (Meloidogyne spp) using monoclonal and polyclonal antibodies ' , Nematologica , vol. 38 , no. 3 , pp. 353-366 .
dc.identifier.issn0028-2596
dc.identifier.otherPURE: 591389
dc.identifier.otherPURE UUID: 601228dd-706d-40fc-a106-eb09132da8d4
dc.identifier.otherWOS: A1992JP36400008
dc.identifier.otherScopus: 0000210460
dc.identifier.otherORCID: /0000-0001-6060-2394/work/32215836
dc.identifier.urihttp://hdl.handle.net/2299/8358
dc.description.abstractPolyclonal and monoclonal antibodies were produced to supernatants of soluble proteins from homogenates of root-knot nematodes (Meloidogyne spp.). The _antibodies were used to investigate differences between second-stage juveniles and adults of the three major root-knot species by ELISA and immunoblotting. Most antigens revealed by the polyclonal antibody were conserved between species but several quantitative differences were observed; large differences were observed between developmental stages. Cluster analysis and principal coordinate analysis using 45 monoclonal antibodies, showing partial discrimination between adult females of the nematode species, divided the nematodes into two groups; one group made up of M. arenaria and a second group composed of M. incognita and M. javanica. Three monoclonal antibodies, exhibiting partial discrimination in the order of producing coefficients of variation between the three species of nematode in excess of 40%, were selected as candidates for a diagnostic assay and immunoblotting analysis. An ELISA based assay using the three monoclonal antibodies in concomitant assays against adult females was able to distinguish the three principal species of root-knot nematodes; the same assay was unable to discriminate between the different species using second-stage juveniles.en
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofNematologica
dc.subjectMELOIDOGYNE
dc.subjectANTIBODIES
dc.subjectIMMUNOLOGICAL DIFFERENTIATION
dc.titleImmunological differentiation of root-knot nematodes (Meloidogyne spp) using monoclonal and polyclonal antibodiesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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