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dc.contributor.authorBrown, Marc
dc.contributor.authorEdmonds, T. E.
dc.contributor.authorMiller, J. N.
dc.contributor.authorRiley, D. P.
dc.contributor.authorSeare, N. J.
dc.date.accessioned2012-04-18T08:57:51Z
dc.date.available2012-04-18T08:57:51Z
dc.date.issued1993-04
dc.identifier.citationBrown , M , Edmonds , T E , Miller , J N , Riley , D P & Seare , N J 1993 , ' Novel instrumentation and biomedical applications of very near-infrared fluorescence ' , Analyst , vol. 118 , no. 4 , pp. 407-410 .
dc.identifier.issn0003-2654
dc.identifier.otherPURE: 631576
dc.identifier.otherPURE UUID: fe5b6752-66ed-43da-a227-4f8cf15ca0c1
dc.identifier.otherWOS: A1993KY80600016
dc.identifier.urihttp://hdl.handle.net/2299/8369
dc.description.abstractA high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha1-acid glycoprotein was achieved with both D,L-propranolol and flufenamic acid, showing that the binding site is less electrostatic and more hydrophobic in nature. In order to monitor these interactions, a simple spectrofluorimeter was constructed from solid-state components; the sensitivity of this instrument compared well with that of standard laboratory spectrofluorimeters.en
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofAnalyst
dc.subjectVERY NEAR INFRARED FLUORESCENCE
dc.subjectSOLID-STATE SPECTROFLUOROMETRY
dc.subjectBIOMEDICAL APPLICATIONS
dc.subjectNILE RED
dc.subjectALPHA-1-ACID GLYCOPROTEIN
dc.subjectHUMAN-SERUM
dc.subjectBINDING
dc.subjectPROBE
dc.subjectSURFACES
dc.subjectALBUMIN
dc.subjectDRUGS
dc.titleNovel instrumentation and biomedical applications of very near-infrared fluorescenceen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
dcterms.dateAccepted1993-04
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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