dc.contributor.author | Brown, Marc | |
dc.contributor.author | Edmonds, T. E. | |
dc.contributor.author | Miller, J. N. | |
dc.contributor.author | Riley, D. P. | |
dc.contributor.author | Seare, N. J. | |
dc.date.accessioned | 2012-04-18T08:57:51Z | |
dc.date.available | 2012-04-18T08:57:51Z | |
dc.date.issued | 1993-04 | |
dc.identifier.citation | Brown , M , Edmonds , T E , Miller , J N , Riley , D P & Seare , N J 1993 , ' Novel instrumentation and biomedical applications of very near-infrared fluorescence ' , Analyst , vol. 118 , no. 4 , pp. 407-410 . | |
dc.identifier.issn | 0003-2654 | |
dc.identifier.uri | http://hdl.handle.net/2299/8369 | |
dc.description.abstract | A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha1-acid glycoprotein was achieved with both D,L-propranolol and flufenamic acid, showing that the binding site is less electrostatic and more hydrophobic in nature. In order to monitor these interactions, a simple spectrofluorimeter was constructed from solid-state components; the sensitivity of this instrument compared well with that of standard laboratory spectrofluorimeters. | en |
dc.format.extent | 4 | |
dc.language.iso | eng | |
dc.relation.ispartof | Analyst | |
dc.subject | VERY NEAR INFRARED FLUORESCENCE | |
dc.subject | SOLID-STATE SPECTROFLUOROMETRY | |
dc.subject | BIOMEDICAL APPLICATIONS | |
dc.subject | NILE RED | |
dc.subject | ALPHA-1-ACID GLYCOPROTEIN | |
dc.subject | HUMAN-SERUM | |
dc.subject | BINDING | |
dc.subject | PROBE | |
dc.subject | SURFACES | |
dc.subject | ALBUMIN | |
dc.subject | DRUGS | |
dc.title | Novel instrumentation and biomedical applications of very near-infrared fluorescence | en |
dc.contributor.institution | Department of Pharmacy | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.description.status | Peer reviewed | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |