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dc.contributor.authorWatt, A.P.
dc.contributor.authorMortishire-Smith, R.J.
dc.contributor.authorGerhard, U.
dc.contributor.authorThomas, S.R.
dc.date.accessioned2012-08-23T14:01:02Z
dc.date.available2012-08-23T14:01:02Z
dc.date.issued2003-01-01
dc.identifier.citationWatt , A P , Mortishire-Smith , R J , Gerhard , U & Thomas , S R 2003 , ' Metabolite identification in drug discovery ' , Current Opinion in Drug Discovery and Development , vol. 6 , no. 1 , pp. 57-65 .
dc.identifier.issn1367-6733
dc.identifier.otherPURE: 940784
dc.identifier.otherPURE UUID: da5a6cf8-0364-4fe4-9916-0468048d4dcb
dc.identifier.otherScopus: 0037277751
dc.identifier.urihttp://hdl.handle.net/2299/8951
dc.descriptionMEDLINE® is the source for the MeSH terms of this document.
dc.description.abstractRecent developments in the technologies and approaches to identify metabolites in a drug discovery environment are reviewed. Samples may be generated using either in vitro systems - typically, but not exclusively, liver subcellular fractions, such as microsomes, or whole cells, such as hepatocytes. Alternatively, metabolites are generated in vivo using excreta obtained following dosing in preclinical species. Recombinant drug metabolizing enzymes or microorganisms may offer alternate vectors. New techniques, such as the use of solid-phase microextraction, have found application in the isolation of metabolites from biological matrices. However, this is still dominated by the use of preparative chromatography, which has advanced through the use of mass-directed detection. Detection and structural elucidation by mass spectrometry have improved markedly with increases in sensitivity, allowing lower abundance metabolites to be detected, and increases in selectivity, with the use of high-resolution time-of-flight and quadrupole-time-of-flight instruments. Finally, higher field strength magnets coupled with novel probe designs and increased use of liquid chromatographic hyphenation techniques continue to drive the capabilities of nuclear magnetic resonance spectroscopy as the definitive structural elucidation tool.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofCurrent Opinion in Drug Discovery and Development
dc.titleMetabolite identification in drug discoveryen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0037277751&partnerID=8YFLogxK
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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