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A hyperspectral fluorescence lifetime probe for skin cancer diagnosis

dc.contributor.authorDe Beule, P A A
dc.contributor.authorDunsby, C
dc.contributor.authorGalletly, N P
dc.contributor.authorStamp, G W
dc.contributor.authorChu, A C
dc.contributor.authorAnand, U
dc.contributor.authorAnand, P
dc.contributor.authorBenham, C.D.
dc.contributor.authorNaylor, A
dc.contributor.authorFrench, P M W
dc.date.accessioned2012-11-14T14:00:03Z
dc.date.available2012-11-14T14:00:03Z
dc.date.issued2007
dc.identifier.citationDe Beule , P A A , Dunsby , C , Galletly , N P , Stamp , G W , Chu , A C , Anand , U , Anand , P , Benham , C D , Naylor , A & French , P M W 2007 , ' A hyperspectral fluorescence lifetime probe for skin cancer diagnosis ' , Review of scientific instruments , vol. 78 , no. 12 , 123101 . https://doi.org/10.1063/1.2818785
dc.identifier.issn0034-6748
dc.identifier.urihttp://hdl.handle.net/2299/9171
dc.description.abstractThe autofluorescence of biological tissue can be exploited for the detection and diagnosis of disease but, to date, its complex nature and relatively weak signal levels have impeded its widespread application in biology and medicine. We present here a portable instrument designed for the in situ simultaneous measurement of autofluorescence emission spectra and temporal decay profiles, permitting the analysis of complex fluorescence signals. This hyperspectral fluorescence lifetime probe utilizes two ultrafast lasers operating at 355 and 440 nm that can excite autofluorescence from many different biomolecules present in skin tissue including keratin, collagen, nicotinamide adenine dinucleotide (phosphate), and flavins. The instrument incorporates an optical fiber probe to provide sample illumination and fluorescence collection over a millimeter-sized area. We present a description of the system, including spectral and temporal characterizations, and report the preliminary application of this instrument to a study of recently resected (en
dc.language.isoeng
dc.relation.ispartofReview of scientific instruments
dc.subjectEquipment Design
dc.subjectEquipment Failure Analysis
dc.subjectHumans
dc.subjectLuminescent Measurements
dc.subjectMolecular Probe Techniques
dc.subjectReproducibility of Results
dc.subjectSensitivity and Specificity
dc.subjectSkin Neoplasms
dc.subjectSpectrometry, Fluorescence
dc.subjectTumor Markers, Biological
dc.titleA hyperspectral fluorescence lifetime probe for skin cancer diagnosisen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionTRP Ion channels
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionBasic and Clinical Science Unit
dc.contributor.institutionCentre for Health Services and Clinical Research
dc.contributor.institutionCentre for Research in Mechanisms of Disease and Drug Discovery
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1063/1.2818785
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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