Show simple item record

dc.contributor.authorDarapaneni, V.
dc.contributor.authorPrabhakar, V.
dc.contributor.authorKukol, A.
dc.date.accessioned2013-01-11T13:29:11Z
dc.date.available2013-01-11T13:29:11Z
dc.date.issued2009-10
dc.identifier.citationDarapaneni , V , Prabhakar , V & Kukol , A 2009 , ' Large-scale analysis of Influenza A virus sequences reveals potential drug-target sites of non-structural proteins ' , Journal of General Virology , vol. 90 , no. 9 , pp. 2124-2133 . https://doi.org/10.1099/vir.0.011270-0
dc.identifier.issn0022-1317
dc.identifier.otherdspace: 2299/4554
dc.identifier.urihttp://hdl.handle.net/2299/9576
dc.descriptionOriginal article can be found at: http://vir.sgmjournals.org/ Copyright Society for General Microbiology "This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription." DOI: 10.1099/vir.0.011270-0
dc.description.abstractThe non-structural protein 1 (NS1) of the influenza A virus and the NS2 protein, which is also known as nuclear export protein, play important roles in the infectious life cycle of the virus. The objective of this study was to find the degree of conservation in the NS proteins and to identify conserved sites of functional or structural importance that may be utilized as potential drug target sites. The analysis was based on 2620 amino acid sequences for the NS1 protein and 1195 sequences for the NS2 protein. The degree of conservation and potential binding sites were mapped onto the protein structures obtained from a combination of experimentally available structure fragments with predicted threading models. In addition to high conservation in protein regions of known function, novel highly conserved sites have been identified, namely Glu159, Thr171, Val192, Arg200, Glu208 and Gln218 on the NS1 protein and Ser24, Leu28, Arg66, Arg84, Ser93, Ile97 and Leu103 on the NS2 protein. Using the Q-SiteFinder binding site prediction algorithm, several highly conserved binding sites were found, including two spatially close sites on the NS1 protein, which could be targeted with a bivalent ligand that would interfere with double-stranded RNA binding. Altogether, this work reveals novel universally conserved residues that are candidates for protein–protein interactions and provide the basis for designing universal anti-influenza drugs.en
dc.format.extent592470
dc.language.isoeng
dc.relation.ispartofJournal of General Virology
dc.subjectinfluenza virus
dc.subjectnon-structural protein
dc.subjectnuclear export protein
dc.subjectbioinformatics
dc.subjectdrug target
dc.subjectbinding site
dc.titleLarge-scale analysis of Influenza A virus sequences reveals potential drug-target sites of non-structural proteinsen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionCentre for Research in Mechanisms of Disease and Drug Discovery
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Future Societies Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1099/vir.0.011270-0
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record