dc.contributor.author | Kukol, A. | |
dc.contributor.author | Li, P. | |
dc.contributor.author | Estrela, P. | |
dc.contributor.author | Ko-Ferrigno, P. | |
dc.contributor.author | Migliorato, P. | |
dc.date.accessioned | 2013-01-11T13:59:03Z | |
dc.date.available | 2013-01-11T13:59:03Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Kukol , A , Li , P , Estrela , P , Ko-Ferrigno , P & Migliorato , P 2008 , ' Label free electrical detection of DNA hybridisation for the example of influenza virus gene sequences ' , Analytical Biochemistry , vol. 374 , no. 1 , pp. 143-153 . https://doi.org/10.1016/j.ab.2007.10.035 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.other | dspace: 2299/1602 | |
dc.identifier.uri | http://hdl.handle.net/2299/9580 | |
dc.description.abstract | Microarrays based on DNA–DNA hybridization are potentially useful for detecting and subtyping viruses but require fluorescence labeling and imaging equipment. We investigated a label-free electrical detection system using electrochemical impedance spectroscopy that is able to detect hybridization of DNA target sequences derived from avian H5N1 influenza virus to gold surface-attached singlestranded DNA oligonucleotide probes. A 23-nt probe is able to detect a 120-nt base fragment of the influenza A hemagglutinin gene sequence. We describe a novel method of data analysis that is compatible with automatic measurement without operator input, contrary to curve fitting used in conventional electrochemical impedance spectroscopy (EIS) data analysis. A systematic investigation of the detection signal for various spacer molecules between the oligonucleotide probe and the gold surface revealed that the signal/background ratio improves as the length of the spacer increases, with a 12- to 18-atom spacer element being optimal. The optimal spacer molecule allows a detection limit between 30 and 100 fmol DNA with a macroscopic gold disc electrode of 1 mm radius. The dependence of the detection signal on the concentration of a 23-nt target follows a binding curve with an approximate 1:1 stoichiometry and a dissociation constant of Kp = 13 ± 4 nM at 295 K. | en |
dc.format.extent | 199350 | |
dc.language.iso | eng | |
dc.relation.ispartof | Analytical Biochemistry | |
dc.title | Label free electrical detection of DNA hybridisation for the example of influenza virus gene sequences | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/j.ab.2007.10.035 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |