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dc.contributor.authorKukol, A.
dc.contributor.authorLi, P.
dc.contributor.authorEstrela, P.
dc.contributor.authorKo-Ferrigno, P.
dc.contributor.authorMigliorato, P.
dc.date.accessioned2013-01-11T13:59:03Z
dc.date.available2013-01-11T13:59:03Z
dc.date.issued2008
dc.identifier.citationKukol , A , Li , P , Estrela , P , Ko-Ferrigno , P & Migliorato , P 2008 , ' Label free electrical detection of DNA hybridisation for the example of influenza virus gene sequences ' , Analytical Biochemistry , vol. 374 , no. 1 , pp. 143-153 . https://doi.org/10.1016/j.ab.2007.10.035
dc.identifier.issn0003-2697
dc.identifier.otherPURE: 121568
dc.identifier.otherPURE UUID: ca0b763a-762f-47fd-a2d2-13660037a463
dc.identifier.otherdspace: 2299/1602
dc.identifier.otherScopus: 38649107404
dc.identifier.urihttp://hdl.handle.net/2299/9580
dc.description.abstractMicroarrays based on DNA–DNA hybridization are potentially useful for detecting and subtyping viruses but require fluorescence labeling and imaging equipment. We investigated a label-free electrical detection system using electrochemical impedance spectroscopy that is able to detect hybridization of DNA target sequences derived from avian H5N1 influenza virus to gold surface-attached singlestranded DNA oligonucleotide probes. A 23-nt probe is able to detect a 120-nt base fragment of the influenza A hemagglutinin gene sequence. We describe a novel method of data analysis that is compatible with automatic measurement without operator input, contrary to curve fitting used in conventional electrochemical impedance spectroscopy (EIS) data analysis. A systematic investigation of the detection signal for various spacer molecules between the oligonucleotide probe and the gold surface revealed that the signal/background ratio improves as the length of the spacer increases, with a 12- to 18-atom spacer element being optimal. The optimal spacer molecule allows a detection limit between 30 and 100 fmol DNA with a macroscopic gold disc electrode of 1 mm radius. The dependence of the detection signal on the concentration of a 23-nt target follows a binding curve with an approximate 1:1 stoichiometry and a dissociation constant of Kp = 13 ± 4 nM at 295 K.en
dc.language.isoeng
dc.relation.ispartofAnalytical Biochemistry
dc.rightsOpen
dc.titleLabel free electrical detection of DNA hybridisation for the example of influenza virus gene sequencesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionCardiovascular Pathologies
dc.contributor.institutionBiochemistry and Bioinformatics
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2008
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.ab.2007.10.035
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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