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dc.contributor.authorKukol, A.
dc.contributor.authorLi, P.
dc.contributor.authorEstrela, P.
dc.contributor.authorKo-Ferrigno, P.
dc.contributor.authorMigliorato, P.
dc.date.accessioned2013-01-11T13:59:03Z
dc.date.available2013-01-11T13:59:03Z
dc.date.issued2008
dc.identifier.citationKukol , A , Li , P , Estrela , P , Ko-Ferrigno , P & Migliorato , P 2008 , ' Label free electrical detection of DNA hybridisation for the example of influenza virus gene sequences ' , Analytical Biochemistry , vol. 374 , no. 1 , pp. 143-153 . https://doi.org/10.1016/j.ab.2007.10.035
dc.identifier.issn0003-2697
dc.identifier.otherPURE: 121568
dc.identifier.otherPURE UUID: ca0b763a-762f-47fd-a2d2-13660037a463
dc.identifier.otherdspace: 2299/1602
dc.identifier.otherScopus: 38649107404
dc.identifier.urihttp://hdl.handle.net/2299/9580
dc.description.abstractMicroarrays based on DNA–DNA hybridization are potentially useful for detecting and subtyping viruses but require fluorescence labeling and imaging equipment. We investigated a label-free electrical detection system using electrochemical impedance spectroscopy that is able to detect hybridization of DNA target sequences derived from avian H5N1 influenza virus to gold surface-attached singlestranded DNA oligonucleotide probes. A 23-nt probe is able to detect a 120-nt base fragment of the influenza A hemagglutinin gene sequence. We describe a novel method of data analysis that is compatible with automatic measurement without operator input, contrary to curve fitting used in conventional electrochemical impedance spectroscopy (EIS) data analysis. A systematic investigation of the detection signal for various spacer molecules between the oligonucleotide probe and the gold surface revealed that the signal/background ratio improves as the length of the spacer increases, with a 12- to 18-atom spacer element being optimal. The optimal spacer molecule allows a detection limit between 30 and 100 fmol DNA with a macroscopic gold disc electrode of 1 mm radius. The dependence of the detection signal on the concentration of a 23-nt target follows a binding curve with an approximate 1:1 stoichiometry and a dissociation constant of Kp = 13 ± 4 nM at 295 K.en
dc.language.isoeng
dc.relation.ispartofAnalytical Biochemistry
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.titleLabel free electrical detection of DNA hybridisation for the example of influenza virus gene sequencesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionCardiovascular Pathologies
dc.contributor.institutionBiochemistry and Bioinformatics
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.ab.2007.10.035
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeclosedAccess


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