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dc.contributor.authorMauchline, Tim H.
dc.contributor.authorKnox, Rachel
dc.contributor.authorMohan, Sharad
dc.contributor.authorPowers, Stephen J.
dc.contributor.authorKerry, Brian R.
dc.contributor.authorDavies, Keith G.
dc.contributor.authorHirsch, Penny R.
dc.date.accessioned2013-01-16T15:29:01Z
dc.date.available2013-01-16T15:29:01Z
dc.date.issued2011-09
dc.identifier.citationMauchline , T H , Knox , R , Mohan , S , Powers , S J , Kerry , B R , Davies , K G & Hirsch , P R 2011 , ' Identification of New Single Nucleotide Polymorphism-Based Markers for Inter- and Intraspecies Discrimination of Obligate Bacterial Parasites (Pasteuria spp.) of Invertebrates ' , Applied and Environmental Microbiology , vol. 77 , no. 18 , pp. 6388-6394 . https://doi.org/10.1128/AEM.05185-11
dc.identifier.issn0099-2240
dc.identifier.otherPURE: 589447
dc.identifier.otherPURE UUID: 0682f33f-7874-4afb-9eca-a0f2567fd81c
dc.identifier.otherWOS: 000294691400009
dc.identifier.otherScopus: 80052819447
dc.identifier.urihttp://hdl.handle.net/2299/9687
dc.description.abstractProtein-encoding and 16S rRNA genes of Pasteuria penetrans populations from a wide range of geographic locations were examined. Most interpopulation single nucleotide polymorphisms (SNPs) were detected in the 16S rRNA gene. However, in order to fully resolve all populations, these were supplemented with SNPs from protein-encoding genes in a multilocus SNP typing approach. Examination of individual 16S rRNA gene sequences revealed the occurrence of "cryptic" SNPs which were not present in the consensus sequences of any P. penetrans population. Additionally, hierarchical cluster analysis separated P. penetrans 16S rRNA gene clones into four groups, and one of which contained sequences from the most highly passaged population, demonstrating that it is possible to manipulate the population structure of this fastidious bacterium. The other groups were made from representatives of the other populations in various proportions. Comparison of sequences among three Pasteuria species, namely, P. penetrans, P. hartismeri, and P. ramosa, showed that the protein-encoding genes provided greater discrimination than the 16S rRNA gene. From these findings, we have developed a toolbox for the discrimination of Pasteuria at both the inter-and intraspecies levels. We also provide a model to monitor genetic variation in other obligate hyperparasites and difficult-to-culture microorganisms.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofApplied and Environmental Microbiology
dc.subjectROOT-KNOT NEMATODES
dc.subjectRIBOSOMAL-RNA GENE
dc.subjectPHYLOGENETIC ANALYSIS
dc.subjectHYPERPARASITIC BACTERIUM
dc.subjectPENETRANS
dc.subjectMELOIDOGYNE
dc.subjectSEQUENCES
dc.subjectPSEUDOMONAS
dc.subjectATTACHMENT
dc.subjectDIVERSITY
dc.titleIdentification of New Single Nucleotide Polymorphism-Based Markers for Inter- and Intraspecies Discrimination of Obligate Bacterial Parasites (Pasteuria spp.) of Invertebratesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionAgriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=80052819447&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2011-09
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1128/AEM.05185-11
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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