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dc.contributor.authorKarunker, Iris
dc.contributor.authorBenting, Juergen
dc.contributor.authorLueke, Bettina
dc.contributor.authorPonge, Tanja
dc.contributor.authorNauen, Ralf
dc.contributor.authorRoditakis, Emmanouil
dc.contributor.authorVontas, John
dc.contributor.authorGorman, Kevin
dc.contributor.authorDenholm, Ian
dc.contributor.authorMorin, Shai
dc.date.accessioned2013-01-16T15:29:12Z
dc.date.available2013-01-16T15:29:12Z
dc.date.issued2008-06
dc.identifier.citationKarunker , I , Benting , J , Lueke , B , Ponge , T , Nauen , R , Roditakis , E , Vontas , J , Gorman , K , Denholm , I & Morin , S 2008 , ' Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera : Aleyrodidae) ' , Insect Biochemistry and Molecular Biology , vol. 38 , no. 6 , pp. 634-644 . https://doi.org/10.1016/j.ibmb.2008.03.008
dc.identifier.issn0965-1748
dc.identifier.otherPURE: 772881
dc.identifier.otherPURE UUID: 9ae85174-03ba-47aa-a7a6-c3d112fa365c
dc.identifier.otherWOS: 000257002900004
dc.identifier.otherScopus: 44249107828
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873714
dc.identifier.urihttp://hdl.handle.net/2299/9689
dc.description.abstractThe two most damaging biotypes of Bemisia tabaci, B and Q, have both evolved strong resistance to the neonicotinoid insecticide imidacloprid. The major mechanism in all samples investigated so far appeared to be enhanced detoxification by cytochrome P450s monooxygenases (P450s). In this study, a polymerase chain reaction (PCR) technology using degenerate primers based on conserved P450 helix I and helne-binding regions was employed to identify P450 cDNA sequences in B. tabaci that might be involved in imidacloprid resistance. Eleven distinct P450 cDNA sequences were isolated and classified as members of the CYP4 or CYP6 families. The mRNA expression levels of all 11 genes were compared by real-time quantitative RT-PCR across nine B and Q field-derived strains of B. tabaci showing strong resistance, moderate resistance or susceptibility to imidacloprid. We found that constitutive overexpression (up to similar to 17-fold) of a single P450 gene, CYP6CM1, was tightly related to imidacloprid resistance in both the B and Q biotypes. Next, we identified three single-nucleotide polymorphic (SNP) markers in the intron region of CYP6CM7 that discriminate between the resistant and susceptible Q-biotype CYP6CM1 alleles (r-Q and s-Q, respectively), and used a heterogeneous strain to test for association between r-Q and resistance. While survivors of a low imidacloprid dose carried both the r-Q and s-Q alleles, similar to 95% of the survivors of a high imidacloprid dose carried only the r-Q allele. Together with previous evidence, the results reported here identify enhanced activity of P450s as the major mechanism of imidacloprid resistance in B. tabaci, and the CYP6CM1 gene as a leading target for DNA-based screening for resistance to imidacloprid and possibly other neonicotinoids in field populations. (c) 2008 Elsevier Ltd. All rights reserved.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofInsect Biochemistry and Molecular Biology
dc.subjectBemisia tabaci
dc.subjectmetabolic resistance
dc.subjectneonicotinoid insecticide
dc.subjectimidacloprid
dc.subjectcytochrome p450s monooxygenases
dc.subjectbiotypes
dc.subjectNEONICOTINOID CROSS-RESISTANCE
dc.subjectPOLYMERASE-CHAIN-REACTION
dc.subjectINSECTICIDE RESISTANCE
dc.subjectMULTIPLE ORIGINS
dc.subjectDROSOPHILA-MELANOGASTER
dc.subjectPYRETHROID RESISTANCE
dc.subjectMETABOLIC RESISTANCE
dc.subjectACCESSION NUMBERS
dc.subjectGENETIC DIVERSITY
dc.subjectP450 SUPERFAMILY
dc.subjectAgricultural and Biological Sciences(all)
dc.titleOver-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera : Aleyrodidae)en
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.ibmb.2008.03.008
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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