Show simple item record

dc.contributor.authorBrzozowski, Monika J.
dc.contributor.authorAlcantara, Susana Lopez
dc.contributor.authorIravani, Mahmoud M.
dc.contributor.authorRose, Sarah
dc.contributor.authorJenner, Peter
dc.date.accessioned2013-02-04T15:00:23Z
dc.date.available2013-02-04T15:00:23Z
dc.date.issued2011-08-02
dc.identifier.citationBrzozowski , M J , Alcantara , S L , Iravani , M M , Rose , S & Jenner , P 2011 , ' The effect of nNOS inhibitors on toxin-induced cell death in dopaminergic cell lines depends on the extent of enzyme expression ' , Brain Research , vol. 1404 , pp. 21-30 . https://doi.org/10.1016/j.brainres.2011.05.063
dc.identifier.issn0006-8993
dc.identifier.otherPURE: 734758
dc.identifier.otherPURE UUID: 40a2d3f6-7954-4c7f-816a-004174786ff8
dc.identifier.otherWOS: 000293489800003
dc.identifier.otherScopus: 79960433284
dc.identifier.otherORCID: /0000-0002-4905-9682/work/32997574
dc.identifier.urihttp://hdl.handle.net/2299/9837
dc.description.abstractNitric oxide is linked with neurodegeneration in Parkinson's disease (PD) through the involvement of both inducible (iNOS) and neuronal nitric oxide synthase (nNOS). While nonselective NOS inhibitors are neuroprotective, the role of nNOS has not been determined using selective NOS inhibitors. The present study investigated the neuroprotective effect of selective iNOS and nNOS inhibitors on MPP+- and MG-132-induced cell death in cell lines with differing levels of nNOS expression. Inhibition of endogenously expressed nNOS by 7-NI and ARR17477 enhanced the toxicity of MPP+ and MG-132 in N1E-115 cells, whereas in transfected SH-SYSY cells overexpressing nNOS, ARR17477 and 7-NI protected against MPP+- and MG-132-induced cell death. In contrast, inhibition of iNOS by 1400W was ineffective in preventing MPP+ and MG-132 toxicity in these cell lines. These results suggest a dual role for NOS in dopaminergic cell viability. nNOS is protective against toxic insult when produced endogenously. When nNOS is overexpressed, it becomes neurotoxic to cells suggesting that inhibition of nNOS may be a promising strategy to prevent cell death in PD. (C) 2011 Elsevier B.V. All rights reserved.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofBrain Research
dc.subjectParkinson's disease
dc.subjectNitric oxide
dc.subjectNeuronal nitric oxide synthase
dc.subjectCell death
dc.subjectDopaminergic cell line
dc.subjectToxin
dc.subjectNITRIC-OXIDE SYNTHASE
dc.subjectSH-SY5Y NEUROBLASTOMA-CELLS
dc.subjectPARKINSONS-DISEASE
dc.subjectMPTP NEUROTOXICITY
dc.subjectTYROSINE-HYDROXYLASE
dc.subject7-NITROINDAZOLE PROTECTS
dc.subjectSERUM DEPRIVATION
dc.subjectCLONE N1E-115
dc.subjectANIMAL-MODELS
dc.subjectUP-REGULATION
dc.titleThe effect of nNOS inhibitors on toxin-induced cell death in dopaminergic cell lines depends on the extent of enzyme expressionen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionNeurodegenerative Diseases
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.brainres.2011.05.063
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record