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dc.contributor.authorMoger, Julian
dc.contributor.authorGarrett, Natalie. L.
dc.contributor.authorBegley, David
dc.contributor.authorMihoreanu, Larisa
dc.contributor.authorLalatsa, Aikaterini
dc.contributor.authorLozano, Maria Victoria
dc.contributor.authorMazza, Mariarosa
dc.contributor.authorSchatzlein, Andreas
dc.contributor.authorUchegbu, Ijeoma
dc.date.accessioned2013-02-11T11:00:27Z
dc.date.available2013-02-11T11:00:27Z
dc.date.issued2012-05
dc.identifier.citationMoger , J , Garrett , N L , Begley , D , Mihoreanu , L , Lalatsa , A , Lozano , M V , Mazza , M , Schatzlein , A & Uchegbu , I 2012 , ' Imaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopy ' , Journal of Raman Spectroscopy , vol. 43 , no. 5 , pp. 668-674 . https://doi.org/10.1002/jrs.3156
dc.identifier.issn0377-0486
dc.identifier.otherPURE: 1268665
dc.identifier.otherPURE UUID: 31ec0904-a232-47ff-b2c4-57b84f1a47dc
dc.identifier.otherWOS: 000304149900013
dc.identifier.otherScopus: 84861348958
dc.identifier.urihttp://hdl.handle.net/2299/9936
dc.description.abstractThe ability to map microvascular morphology and hemodynamic parameters, such as blood volume, is desirable for many biomedical studies and will lead to a deeper understanding of the mechanisms of angiogenesis and vascular disease. Capillary networks can be delineated in three dimensions with two-photon excited fluorescence microscopy; however, this requires the intravenous infusion of a fluorescent dye into the blood plasma, which often complicates in vivo imaging and only provides an indirect estimate of local haematocrit volume. Moreover, visualising the spatial distribution of capillaries is often insufficient; ideally, one would wish to correlate the proximity of the blood vessels with the surrounding local tissue structure. We present in this study a novel multimodal approach that combines stimulated Raman scattering and two-photon photothermal lensing to provide simultaneous visualisation of cortical microvasular morphology and surrounding cellular structures. We show that volumetric analysis of the nonlinear photothermal contrast of erythrocytes allows a direct quantification of local haematocrit volume rather than relying upon average plasma volume-to-haematocrit ratios. Copyright (c) 2012 John Wiley & Sons, Ltd.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofJournal of Raman Spectroscopy
dc.titleImaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopyen
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutics
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1002/jrs.3156
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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