The isolation and culture of cucumber mesophyll protoplasts

Coutts, Robert H.A. and Wood, K.R. (1975) The isolation and culture of cucumber mesophyll protoplasts. Plant Science Letters (3). pp. 189-193. ISSN 0304-4211
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Large numbers of viable protoplasts were obtained from the first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China. Cell wall regeneration was observed in liquid culture within 3 days of isolation, and division within 5 days

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