Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells

Njoh, Kerenza L., Patterson, Laurence H., Zloh, Mire, Wiltshire, Marie, Fisher, Janet, Chappell, Sally, Ameer-Beg, Simon, Bai, Yanhong, Matthews, Daniel, Errington, Rachel J. and Smith, Paul J. (2006) Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells. Cytometry Part A (8). pp. 805-14. ISSN 1552-4922
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We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.

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