Negatively supercoiled DNA from plants infected with a single-stranded DNA virus
Sunter, G., Coutts, Robert H.A. and Buck, K.W.
(1984)
Negatively supercoiled DNA from plants infected with a single-stranded DNA virus.
Biochemical and Biophysical Research Communications (3).
pp. 747-752.
ISSN 0006-291X
A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme
| Item Type | Article |
|---|---|
| Date Deposited | 29 May 2025 09:11 |
| Last Modified | 29 May 2025 09:11 |