Multiple forms of bovine pepsinogen : isolation and identification in serum from calves with ostertagiasis

By using fast protein liquid chromatography (FPLC) a new form of bovine pepsinogen has been identified. Compared with the previously isolated pepsinogen (PG I) the new pepsinogen (PG II) is less mobile on agarose electrophoresis, is eluted earlier from an anion-exchange column and is 30 times less abundant. Agarose electrophoresis identified PG I in the serum of calves given third stage Ostertagia ostertagi larvae, while FPLC identified both PG I and PG II in such serum.