Use of a cellulose-acetate electrophoresis system for the simultaneous characterization of individual root-knot (meloidogyne spp) females using 3 isozymes

A cellulose acetate electrophoresis system is described which can be used for the identification of root-knot nematodes on a routine basis. The results of staining for several isozymes (EST, PGI, MDH & PGM) is reported. It was possible, with large healthy females of root-knot nematodes, to analyse up to three different isozymes per individual female. The technique is briefly discussed in comparison to DNA based approaches for root-knot identification