Novel instrumentation and biomedical applications of very near-infrared fluorescence

Brown, Marc, Edmonds, T. E., Miller, J. N., Riley, D. P. and Seare, N. J. (1993) Novel instrumentation and biomedical applications of very near-infrared fluorescence. Analyst (4). pp. 407-410. ISSN 0003-2654
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A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha1-acid glycoprotein was achieved with both D,L-propranolol and flufenamic acid, showing that the binding site is less electrostatic and more hydrophobic in nature. In order to monitor these interactions, a simple spectrofluorimeter was constructed from solid-state components; the sensitivity of this instrument compared well with that of standard laboratory spectrofluorimeters.

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