Novel instrumentation and biomedical applications of very near-infrared fluorescence
Brown, Marc, Edmonds, T. E., Miller, J. N., Riley, D. P. and Seare, N. J.
(1993)
Novel instrumentation and biomedical applications of very near-infrared fluorescence.
Analyst (4).
pp. 407-410.
ISSN 0003-2654
A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha1-acid glycoprotein was achieved with both D,L-propranolol and flufenamic acid, showing that the binding site is less electrostatic and more hydrophobic in nature. In order to monitor these interactions, a simple spectrofluorimeter was constructed from solid-state components; the sensitivity of this instrument compared well with that of standard laboratory spectrofluorimeters.
Item Type | Article |
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Keywords | VERY NEAR INFRARED FLUORESCENCE; SOLID-STATE SPECTROFLUOROMETRY; BIOMEDICAL APPLICATIONS; NILE RED; ALPHA-1-ACID GLYCOPROTEIN; HUMAN-SERUM; BINDING; PROBE; SURFACES; ALBUMIN; DRUGS |
Date Deposited | 29 May 2025 09:14 |
Last Modified | 29 May 2025 09:14 |