Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology
The sequence of the four large Q double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACID L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACID L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACID L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACID L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.
Item Type | Article |
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Keywords | GENOME; HELICOBASIDIUM-MOMPA TANAKA; PLANT-PATHOGENIC FUNGUS; CHLOROTIC RUSTY SPOT; DISORDER; VIRUS; SEQUENCE ALIGNMENT; COMPLEX PATTERN; POLYMERASES |
Date Deposited | 29 May 2025 09:15 |
Last Modified | 29 May 2025 09:15 |