The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles
Aims To demonstrate the potential use of in vitro poly (lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. Methods PLGA microparticles (diameter 10-60 mu m) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. Results Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. Conclusion The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.
Item Type | Article |
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Additional information | This paper is freely available online under the BMJ Journals unlocked scheme, see http:// bjo.bmj.com/site/about/unlocked.xhtm |
Keywords | preferred substrate sequences, displayed peptide library, triamcinolone acetonide, anterior-chamber, microbial transglutaminase, tissue transglutaminase, cataract-surgery, microspheres, capsule, cells |
Date Deposited | 15 May 2025 12:42 |
Last Modified | 30 May 2025 23:56 |
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