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Browsing by Author "Coutts, Robert H.A."
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Current evidence and future perspectives on HuR and breast cancer development, prognosis, and treatment.
Kotta-Loizou, Ioly; Vasilopoulos, Spyridon N.; Coutts, Robert H.A.; Theocharis, Stamatios (2016-11-01)Hu-antigen R (HuR) is an RNA-binding posttranscriptional regulator that belongs to the Hu/ELAV family. HuR expression levels are modulated by a variety of proteins, microRNAs, chemical compounds, or the microenvironment, ... -
Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components
Hamilton, W.D.O.; Bisaro, D.M.; Coutts, Robert H.A.; Buck, K.W. (1983-11-11)Linear double-stranded (ds)DNA, obtained by excision of the cloned A and B components of tomato golden mosaic virus (TGMV) from recombinant plasmids, was found to infect plants and to elicit symptoms identical to those ... -
Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease
Kozlakidis, Z.; Citir, A.; Acikgoz, S.; Coutts, Robert H.A. (2007-12)A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based ... -
Differentiation between cucurbit yellow stunting disorder virus and beet pseudo-yellows virus by a reverse transcription-polymerase chain reaction assay
Livieratos, I.C.; Coutts, Robert H.A.; Katis, N. (1998-06)Two whitefly-transmitted viruses elicit identical symptoms of interveinal chlorosis (yellowing) in greenhouse- and field-grown cucurbits in Mediterranean countries. The agents of these diseases are partially characterized ... -
Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons
Kulshreshtha, S.; Coutts, Robert H.A. (1997)An in vitro protocol has been developed for direct somatic embryogenesis of zygotic cotyledons from mature sugarbeet (Beta vulgaris L.) embryos. Explants were sequentially cultured on modified Murashige and Skoog (MS) ... -
Diversity of Burkholderia isolates from woodland rhizosphere environments
Richardson, J.; Stead, D.E.; Elphinstone, J.G.; Coutts, Robert H.A. (2002-10)Aims: Determination of genetic diversity among UK Burkholderia cepacia isolates from various environmental niches, principally woodland tree rhizospheres and onions. Methods and Results: Genus determination was made using ... -
DNA and RNA polymerase activities of nuclei and hypotonic extracts of nuclei isolated from tomato golden mosaic virus infected tobacco leaves
Coutts, Robert H.A.; Buck, K.W. (1985-11-11)Nuclei and hypotonically leached extracts of nuclei prepared from tomato golden mosaic virus (TGMV)-infected Nicotiana benthamiana leaves have been used in in vitro DNA and RNA polymerisation reactions. The synthesis of ... -
DsRNA cloning and diagnosis of beet pseudo-yellows virus by PCR and nucleic acid hybridization
Coffin, R.S.; Coutts, Robert H.A. (1992)DsRNA has been extracted from beet pseudo-yellows virus infected cucumber plants, purified to homogeneity, and cDNA clones to it produced. The clones are of insufficient sensitivity to detect infection-specific RNA in dot ... -
Early stages in cell wall regeneration of tobacco mesophyll protoplasts : An electrophoretic study
Coutts, Robert H.A.; Grout, B.W.W. (1975-02-01)The electrophoretic mobilities of isolated mesophyll protoplasts of Nicotiana tabacum var. Xanthi n.c. were measured using a whole cell electrophoresis apparatus. Changes in mobility were recorded at times during aseptic ... -
The effects of dsRNA mycoviruses on growth and murine virulence of Aspergillus fumigatus
Bhatti, Muhammad F.; Jamal, Atif; Petrou, Michael A.; Cairns, Timothy C.; Bignell, Elaine M.; Coutts, Robert H.A. (2011-11)Some isolates of the opportunistic human pathogenic fungus Aspergillus fumigatus are known to be infected with mycoviruses. The dsRNA genomes of two of these mycoviruses, which include a chrysovirus and a partitivirus, ... -
Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture
Adejare, G.O.; Coutts, Robert H.A. (1981-01)Methods previously established for the propagation of cassava plants free from cassava mosaic disease have been applied to Nigerian clones. Meristem tips from diseased plants subjected to heat treatment for not less than ... -
Erratum to: The complete nucleotide sequence of a totivirus from Aspergillus foetidus (Arch Virol, 10.1007/s00705-012-1368-x)
Kozlakidis, Z.; Herrero, N.; Coutts, Robert H.A. (2013-02) -
The family Closteroviridae revised
Martelli, G.P.; Agranovsky, A.A.; Bar-Joseph, M.; Boscia, D.; Minafra, A.; Candresse, T.; Coutts, Robert H.A.; Dolja, V.V.; Falk, B.W.; Gonsalves, D.; Jelkmann, W.; Karasev, A.V.; Namba, S.; Vetten, H.J.; Wisler, G.C.; Yoshikawa, N. (2002-10)Recently obtained molecular and biological information has prompted the revision of the taxonomic structure of the family Closteroviridae. In particular, mealybug-transmitted species have been separated from the genus ... -
First report of an Endornavirus in the Cucurbitaceae
Coutts, Robert H.A. (2005-12-01) -
Geminivirus coat protein gene promoter sequence can function in Escherichia coli
Petty, I.T.D.; Coutts, Robert H.A.; Buck, K.E. (1986-06-25) -
Gene amplification and expression in plants by a replicating geminivirus vector
Hayes, R.J.; Petty, I.T.D.; Coutts, Robert H.A.; Buck, K.W. (1988-01-01)The use of plant DNA viruses as vectors for the transfer of foreign genes to plants offers two potential advantages over other, existing methods of producing transgenic plants. First, these viruses sys-temically infect ... -
Identification and sequence analysis of Potato yellow vein virus capsid protein minor gene
Livieratos, I.C.; Muller, G.; Salazar, L.F.; Eliasco, E.; Coutts, Robert H.A. (2002-12)Potato yellow vein virus (PYVV) is a whitefly-transmitted (Trialeurodes vaporariorum) closterovirus (WTC) with an as yet unidentified genome composition. PYVV dsRNA preparations consist of three high molecular weight dsRNA ... -
Identification and sequence determination of a novel double-stranded RNA mycovirus from the entopathogenic fungus Beauveria bassina
Kotta-Loizou, I.; Sipkova, J.; Coutts, Robert H.A. (2015-03-31)An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five double-stranded (ds) RNA elements ranging from 1.5 to more than 3 kbp. The complete sequence of the largest dsRNA element is described ... -
Improved isolation and culture methods for cucumber mesophyll protoplasts
Coutts, Robert H.A.; Wood, K.R. (1977-05-01)Improved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods ... -
In vitro synthesis of minus-strand RNA by an isolated cereal yellow dwarf virus RNA-dependent RNA polymerase requires VPg and a stem-loop structure at the 3' end of the virus RNA
Osman, Toba A. M.; Coutts, Robert H.A.; Buck, Kenneth W. (2006-11)Cereal yellow dwarf virus (CYDV) RNA has a 5'-terminal genome-linked protein (VPg). We have expressed the VPg region of the CYDV genome in bacteria and used the purified protein (bVPg) to raise an antiserum which was able ...