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Browsing by Author "Coutts, Robert H.A."
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In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts.
Owen, Carolyn; Moukarzel, Romy; Huang, Xiao; Kassem, Mona; Eliasco, Eleonora; Aranda, Miguel; Coutts, Robert H.A.; Livieratos, Ioannis (2016-06-14)Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA ... -
Incidence of dsRNA mycoviruses in a collection of Aspergillus fumigatus isolates
Bhatti, Muhammad F.; Jamal, Atif; Bignell, Elaine M.; Petrou, Michael A.; Coutts, Robert H.A. (2012-10)A collection of clinical and environmental isolates of the opportunistic human pathogen, Aspergillus fumigatus, were screened for the presence of mycoviruses and 6.6 % of 366 isolates contained dsRNA segments ranging in ... -
Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum
Kozlakidis, Zisis; Brown, Neil A; Jamal, Atif; Phoon, Xiyu; Coutts, Robert H.A. (2010-02)In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates ... -
Incidence of Phlebiopsis gigantea large virus-1 in a collection of Phlebiopsis gigantea isolates
Lim, Jia M.; Jamal, Atif; Phoon, Xiyu; Korhonen, Kari; Coutts, Robert H.A. (2011-11)Eighty six Phlebiopsis gigantea isolates from at least 9 different tree species from various locations in 12 different European countries and North America were screened for the presence of large molecular weight dsRNA >10 ... -
Incidence of the cblA major subunit pilin gene amongst Burkholderia species
Richardson, J.; Stead, D.E.; Coutts, Robert H.A. (2001-03-01)PCR-amplification has been used to screen 75 isolates of the Burkholderia cepacia complex for the cblA pilin gene. PCR-amplified products of the correct size (664 bp) were cloned and sequenced and the sequences compared. ... -
The infection of cucumber mesophyll protoplasts with tobacco mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1976)Protoplasts from the first leaf mesophyll of cucumber plants were isolated by an 18 hour combined petinase/cellulase treatment. Conditions favouring the infection of these protoplasts with tobacco mosaic virus (TMV), and ... -
Infection of protoplasts from yeast with tobacco mosaic virus
Coutts, Robert H.A.; Cocking, E.C.; Kassanis, B. (1972-12-22)PROTOPLASTS from higher plant cells can become infected with plant viruses under special conditions, and it has been shown that yeast cells can become infected with viruses normally found in different fungal genera . We ... -
The infectivity of dimeric potato yellow mosaic geminivirus clones in different hosts
Buragohain, A.K.; Sung, Y.K.; Coffin, R.S.; Coutts, Robert H.A. (1994-10)Head-to-tail dimeric clones of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV) were constructed. These constructs were infectious when inoculated onto Nicotiana benthamiana plants either as DNA or by ... -
Inoculation of leaf mesophyll protoplasts from a resistant and a susceptible cucumber cultivar with cucumber mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1977-01) -
Investigations on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus
Coutts, Robert H.A.; Wood, K.R. (1976)Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts ... -
Investigations on the Tobacco necrosis virus D p60 replicase protein
Fang, Liang; Coutts, Robert H.A. (2013-11-21)Tobacco necrosis virus D (TNV-D), in the genus Betanecrovirus (family Tombusviridae), possesses a single-stranded, positive-sense RNA genome containing six open reading frames (ORFs). Two 5'-proximal ORFs (1 and 2) encode ... -
The isolation and culture of cucumber mesophyll protoplasts
Coutts, Robert H.A.; Wood, K.R. (1975-03-01)Large numbers of viable protoplasts were obtained from the first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China. Cell wall regeneration was observed in liquid culture within 3 days of isolation, ... -
Isolation of an RNA-dependent RNA polymerase from Nicotiana benthamiana plants infected with Tobacco necrosis virus (strain D) and template-dependent transcription of its satellite, Satellite tobacco necrosis virus (strain C) RNA
Coutts, Robert H.A.; Plante, C.A. (2001-04-01)Partially purified RNA-dependent RNA polymerase activity isolated from Nicotiana benthamiana plants infected with Tobacco necrosis virus strain D (TNV-D) is capable of template-dependent synthesis of Satellite tobacco ... -
Location of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAs
Offei, S.K.; Coutts, Robert H.A. (1996-01)Northern blot analysis of double-stranded (ds) RNA from bean-leaf tissue infected with tobacco necrosis virus strain D (TNV-D) detected the 4 kb genomic RNA and two subgenomic RNAs of about 1.5 kb and 1.2 kb; RNA extracted ... -
A modified procedure for isolating double-stranded RNA: Application to diagnosis of Amasya cherry disease
Acikgoez, S.; Doeken, M. T.; Degirmenci, N. F.; Coutts, Robert H.A.; Kozlakidis, Z. (2007-12)A novel method of isolating double-stranded RNA (dsRNA) from cherry leaves affected with Amasya cherry disease (ACD) using silica capture in combination with non-ionic cellulose was developed. Unfractionated RNAs prepared ... -
Molecular and biological characterization of a novel partitivirus from Talaromyces pinophilus
Hassan, Sidra; Syun-ichi, Urayama; Shabeer, Saba; Kiran, Tahseen Ali; Wu, Chien-Fu; Moriyama, Hiromitsu; Coutts, Robert H.A.; Kotta Loizou, Ioly; Jamal, Atif (2024-05)Talaromyces spp. have a worldwide distribution, are ecologically diverse and have been isolated from numerous different substrates. Talaromyces spp. are considered biotechnologically important due to their ability to produce ... -
Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus
Macdowell, S.W.; Coutts, Robert H.A.; Buck, K.W. (1986-10-01)A subgenomic single-stranded DNA present in particles of the geminivirus, tomato golden mosaic virus, has been shown by electron microscope heteroduplex mapping and Southern hybridisation analysis to consist of circular ... -
Molecular characterisation of two novel double-stranded RNA elements from Phlebiopsis gigantea
Kozlakidis, Zisis; Hacker, Caroline V.; Bradley, Dominic; Jamal, Atif; Phoon, Xiyu; Webber, Joan; Brasier, Clive M.; Buck, Kenneth W.; Coutts, Robert H.A. (2009-08)The incomplete sequences of two large, 10-12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode ... -
Molecular characterization of a partitivirus from Ophiostoma himal-ulmi
Crawford, L.J.; Osman, T.A.M.; Coutts, Robert H.A.; Buck, K.W.; Booy, F.P.; Brasier, C.M. (2006-08)The complete nucleotide sequences of two double-stranded (ds) RNA molecules, S1 (1,744 bp) and S2 (1,567 bp), isolated from an isolate HP62 of the Himalayan Dutch elm disease fungus, Ophiostoma himal-ulmi, were determined. ... -
Molecular characterization of a totivirus and a partitivirus from the genus Ophiostoma
Doherty, M.; Sanganee, K.; Kozlakidis, Z.; Coutts, Robert H.A.; Brasier, C. M.; Buck, K. W. (2007-03)Two fungal isolates of the genus Ophiostoma (O. minus and O. quercus) contain double-stranded RNA elements which, when partially cloned and sequenced, represent fragments of RNA-dependent RNA polymerase genes which are ...