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Browsing by Author "Buck, Kenneth W."
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In vitro synthesis of minus-strand RNA by an isolated cereal yellow dwarf virus RNA-dependent RNA polymerase requires VPg and a stem-loop structure at the 3' end of the virus RNA
Osman, Toba A. M.; Coutts, Robert H.A.; Buck, Kenneth W. (2006-11)Cereal yellow dwarf virus (CYDV) RNA has a 5'-terminal genome-linked protein (VPg). We have expressed the VPg region of the CYDV genome in bacteria and used the purified protein (bVPg) to raise an antiserum which was able ... -
Molecular characterisation of two novel double-stranded RNA elements from Phlebiopsis gigantea
Kozlakidis, Zisis; Hacker, Caroline V.; Bradley, Dominic; Jamal, Atif; Phoon, Xiyu; Webber, Joan; Brasier, Clive M.; Buck, Kenneth W.; Coutts, Robert H.A. (2009-08)The incomplete sequences of two large, 10-12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode ...