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Browsing by Author "Hoffman, Ewelina"
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Comparison of Oral, Intranasal and Aerosol Administration of Amiodarone in Rats as a Model of Pulmonary Phospholipidosis.
Bodhaniya, Aateka; Hoffman, Ewelina; Ball, Dough; Klapwijk, Jan; Steven, Rory T.; Dexter, Alex; Bunch, Josephine; Baker, Daniel; Murnane, Darragh; Hutter, Victoria; Page, Clive; Dailey, Lea Ann; Forbes, Ben (2019-07-17)‘Foamy’ alveolar macrophages (FAM) observed in nonclinical toxicology studies during inhaled drug development may indicate drug-induced phospholipidosis, but can also derive from adaptive non-adverse mechanisms. Orally ... -
High content analysis of in vitro alveolar macrophage responses can provide mechanistic insight for inhaled product safety assessment
Hutter, Victoria; Hopper, Sarah; Skamarauskas, John; Hoffman, Ewelina (2023-02-28)Assessing the safety of inhaled substances in the alveolar region of the lung requires an understanding of how the respired material interacts with both physical and immunological barriers. Human alveolar-like macrophages ... -
High Content Image Analysis as a Tool to Morphologically Distinguish Macrophage Activation and Determine Its Importance for Foamy Alveolar Macrophage Responses
Hoffman, Ewelina; Napieralska, Paulina; Mahendran, Rhamiya; Murnane, Darragh; Hutter, Victoria (2021-09-01)Introduction: Lung diseases are an increasing global health burden affecting millions of people worldwide. Only a few new inhaled medicines have reached the market in the last 30 years, in part due to foamy alveolar ... -
Investigating the suitability of high content image analysis as a tool to assess the reversibility of foamy alveolar macrophage phenotypes in vitro.
Hoffman, Ewelina; Murnane, Darragh; Hutter, Victoria (2020-03-13)Many potential inhaled medicines fail during development due to the induction of a highly vacuolated or “foamy” alveolar macrophage phenotype response in pre-clinical studies. There is limited understanding if this response ... -
Longitudinal characterization of TK6 cells sequentially adapted to animal product-free, chemically defined culture medium: considerations for genotoxicity studies
Perez-Diaz, Noelia; Hoffman, Ewelina; Clements, Julie; Cruickshank, Rebecca; Doherty, Ann; Ebner, Daniel; Elloway, Joanne; Fu, Jianan; Kelsall, Joanne; Millar, Val; Saib, Ouarda; Scott, Andrew; Woods, Ian; Hutter, Victoria (2023-07-04)Introduction: In vitro approaches are an essential tool in screening for toxicity of new chemicals, products and therapeutics. To increase the reproducibility and human relevance of these in vitro assessments, it is advocated ... -
Morphometric Characterization of Rat and Human Alveolar Macrophage Cell Models and their Response to Amiodarone using High Content Image Analysis
Hoffman, Ewelina; Bodhaniya, Aateka; Ball, Doug; Klapwijk, Jan; Millar, Val; Kumar, Abhinav; Martin, Abigail; Mahendran, Rhamiya; Dailey, Lea Ann; Forbes, Ben; Hutter, Victoria (2017-12-31)Purpose. Progress to the clinic may be delayed or prevented when vacuolated or “foamy” alveolar macrophages are observed during non-clinical inhalation toxicology assessment. The first step in developing methods to study ... -
Polymer Architecture Effects on Poly(N,N‐Diethyl Acrylamide)‐b‐Poly(Ethylene Glycol)‐b‐Poly(N,N‐Diethyl Acrylamide) Thermoreversible Gels and Their Evaluation as a Healthcare Material
Haddow, Peter J.; da Silva, Marcelo A.; Kaldybekov, Daulet B.; Dreiss, Cecile A.; Hoffman, Ewelina; Hutter, Victoria; Khutoryanskiy, Vitaliy V.; Kirton, Stewart B.; Mahmoudi, Najet; McAuley, William J.; Cook, Michael T. (2021-12-11)Thermoreversible gels which transition between liquid‐like and solid‐like states when warmed have enabled significant novel healthcare technologies. Poly(N,N‐diethyl acrylamide) (PDEA) is a thermoresponsive polymer which ... -
Predicting Skin Permeability by means of Computational Approaches : Reliability and Caveats in Pharmaceutical Studies
Pecoraro, Beatrice; Tutone, Marco; Hoffman, Ewelina; Hutter, Victoria; Almerico, Anna Maria; Traynor, Matthew (2019-05-28)The skin is the main barrier between the internal body environment and the external one. The characteristics of this barrier and its properties are able to modify and affect drug delivery and chemical toxicity parameters. ... -
Profiling alveolar macrophage responses to inhaled compounds using in vitro high content image analysis
Hoffman, Ewelina; Urbano, Laura; Martin, Abigail; Mahendran, Rhamiya; Patel, Aateka; Murnane, Darragh; Page, Clive; Dailey, Lea Ann; Forbes, Ben; Hutter, Victoria (2023-07-12)One of the main hurdles in the development of new inhaled medicines is the frequent observation of foamy macrophage (FM) responses in non-clinical studies in experimental animals, which raises safety concerns and hinders ... -
A quantitative structure-permeability relationship model for split-thickness skin absorption, reasoning for the choice of the database.
Pecoraro, Beatrice; Hoffman, Ewelina; Hutter, Victoria; Traynor, Matthew (2018-06-20)The skin is the largest organ in the human body, protecting the body from xenobiotic invasion (1). Local and systemic drugs may also be administered through the skin, therefore the need to measure the permeability of the ...