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Changes in the Iron Coordination Sphere of Fe(II) Lipoxygenase-1 from Soybeans upon Binding of Linoleate or Oleate
Van Der Heijdt, L.M.; Schilstra, M.; Feiters, M.C.; Navaratnam, S.; Nolting, H-F.; Hermes, C.; Veldink, G.A.; Vliegenthart, J.F.G. (1995)Fe K-edge X-ray absorption spectra of the non-heme iron constituent of lipoxygenase-1 from soybeans were obtained. The spectrum of 2.5 mM Fe(II) lipoxygenase, mixed with 1.2 M linoleate in the absence of O2, was compared ... -
The Dioxygenation Rate in Lipoxygenase Catalysis Is Determined by the Amount of Iron( HI) Lipoxygenase in Solution
Schilstra, M.; Veldink, G.A.; Vliegenthart, J.F.G. (1994) -
Effect of lipid hydroperoxide on lipoxygenase kinetics
Schilstra, M.; Veldink, G.A.; Verhagen, J.; Vliegenthart, J.F.G. (1992)In order to investigate the activation of lipoxygenase and to clarify the role of the oxygenation product hydroperoxide in this process, the effect of 13-hydroperoxylinoleic acid (P, 0-35 pM) on linoleic acid (S, 1-80 pM) ... -
Effect of Nonionic Detergents on Lipoxygenase Catalysis
Schilstra, M.; Veldink, G.A.; Vliegenthart, J.F.G. (1994)In many studies on lipoxygenase catalysis, nonionic detergents are used to obtain an optically transparent solution of the fatty acid substrate. In order to resolve some controversies that exist with regard to the ... -
Fe(II1)-Lipoxygenase Converts Its Suicide-Type Inhibitor Octadeca-9,12-diynoic Acid into 1l -Oxooctadeca-9,12-diynoic Acid
Nieuwenhuizen, W.F.; Schilstra, M.; Van der Kerk, A.; Brandsma, L.; Veldink, G.A.; Vliegenthart, J.F.G. (1995) -
Kinetic analysis of the induction period in lipoxygenase catalysis
Schilstra, M.; Veldink, G.A.; Vliegenthart, J.F.G. (1993)The dioxygenation of 50 pM linoleate at 0.1 pM (13s)-hydroperoxylinoleate, 240 pM 02, pH 10, and 25 O C , catalyzed by varying amounts of soybean lipoxygenase-1, was studied with rapid kinetic techniques. The aim was to ... -
Mechanism of Lipoxygenase Inactivation by the Linoleic Acid Analogue Octadeca-9,12-diynoic Acid
Schilstra, M.; Nieuwenhuizen, W.F.; Veldink, G.A.; Vliegenthart, J.F.G. (1996)During the irreversible inactivation of soybean Fe(III)-lipoxygenase [Fe(III)-LOX] by octadeca-9,12-diynoic acid (ODYA), significant quantities of 11-oxooctadeca-9,12-diynoic acid (11-oxo-ODYA) are formed [Nieuwenhuizen, ... -
Substrates and products in lipoxygenase biocatalysis
Veldink, G.A.; Nieuwenhuizen, W.F.; Schilstra, M.; Vliegenthart, J.F.G. (Friedrich Vieweg & Sons Verlagsgesellschaft mbH, 1995)